Abstract
In the human kidney, binding sites for the alpha1-adrenoceptor antagonist3H-prazosin and the alpha2-adrenoceptor antagonist3H-rauwolscine were localized and quantified byin vitro autoradiography.3H-prazosin binding was found predominantly in the renal cortex. In the medulla, tubular structures were also specifically labelled. No binding sites, however, were detected in association with glomeruli or large blood vessels.3H-rauwolscine labelled the medullary vascular bundles intensively, but no binding sites were associated with glomeruli or other cortical structures. Thus, the binding pattern for3H-prazosin is quite similar in both human and rat renal cortex. There are, however, distinct differences between human and rat kidneys in the distribution of the alpha2-adrenergic binding sites visualized with the antagonist3H-rauwolscine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Summers, R. J.: Renal α-adrenoceptors.Fed. Proc., 43, 2917 (1984).
DiBona, G. F.: Neural control of renal function: Role of renal alpha adrenoceptors.J. Cardiovasc. Pharmacol., 7 (Suppl. 8), S18 (1985).
Strandhoy, J. W.: Role of alpha-2-receptors in the regulation of renal function.J. Cardiovasc. Pharmacol., 7 (Suppl. 8), S28 (1985).
Pettinger, W. A., Umemura, S., Smyth, D. D., Jeffrés, E. B.: Renal α2-adrenoceptors and the adenylate cyclase-cAMP system: Biochemical and physiological interactions.Am. J. Physiol., 252, F199 (1987).
Michel, M. C., Brodde, O. E., Insel, P. A.: Peripheral adrenergic receptors in hypertension.Hypertension, 16, 107 (1990).
Neylon, C. B., Summers, R. J.:3H-Rauwolscine binding to α2-adrenoceptors in the mammalian kidney: Apparent receptor heterogeneity between species.Br. J. Pharmacol., 85, 349 (1985).
Stephenson, J. A., Summers, R. J.: Autoradiographic evidence for a heterogeneous distribution of α1-adrenoceptors labeled by3H-prazosin in rat, dog and human kidney.J. Auton. Pharmacol., 6, 109 (1986).
Umemura, S., Yasuda, G., Uchino, K., Shindo, T., Ischikawa, Y., Toya Kaneko, Y.: Existence of renal α1- and α2-adrenoceptors in the human kidney: Radioligand binding studies in membranes from the human renal cortex and medulla.J. Hyperten., 4 (Suppl. 6), S222 (1986).
Michel, M. C., Brodde, O. E., Schnepel, B., Behrendt, J., Tschada, R., Motalsky, H. J., Insel, P. A.: [3H] idaxozan and some other α2-adrenergic drugs bind with high affinity to a nonadrenergic site.Mol. Pharmacol., 35, 324 (1989).
Dashwood, M. R.: Autoradiographic localization of prazosin binding sites in rat kidney.Eur. J. Pharmacol., 94, 163 (1983).
Muntz, K. H., Garcia, C., Hagler, K. H.: α1-Receptor localization in rat heart and kidney using autoradiography.Am. J. Physiol., 249, H512 (1985).
Wilson, S. K.: Peripheralalpha-1 andalpha-2 adrenergic receptors in three models of hypertension in rats: Anin vitro autoradiographic study.J. Pharmacol. Exp. Ther., 256, 801 (1991).
Muntz, K. H., Meyer, L., Gradol, S., Calianos, T. A.: Alpha-2-adrenergic receptor localization in the rat heart and kidney using autoradiography and tritiated rauwolscine.J. Pharmacol. Exp. Ther., 236, 542 (1986).
Geary, W. A., Toga, A. W., Wooten, G. F.: Quantitative film autoradiography for tritium: Methodological considerations.Brain Res., 337, 99 (1985).
Munson, P. J., Rodbard, D.: LIGAND: A versatile computerized approach for characterization of ligand-binding systems.Anal. Biochem., 107, 220 (1980).
Summers, R. J., Stephenson, A., Lipe, S., Neylon, C. B.: α2-adrenoceptors in dog kidney: Autoradiographic localization and putative functions.Clin. Sci.,68 (Suppl. 10), 105s (1985).
Zilles, K., zur Nieden, K., Schleicher, A., Traber, J.: A new method for quenching correction leads to revision of data in receptor autoradiography.Histochemistry, 94, 569 (1990).
Snavely, M. D., Insel, P. A.: Characterization of alpha-adrenergic receptor subtypes in the rat renal cortex.Mol. Pharmacol., 22, 532 (1982).
Bylund, D. B.: Subtypes of α2-adrenoceptors: Pharmacological and molecular biological evidence converge.Trends Pharmacol. Sci., 9, 356 (1988).
Bylund, D. B., Ray-Prenger, C.: Alpha-2A and alpha-2B adrenergic subtypes: Attenuation of cyclic AMP production in cell lines containing only one receptor subtype.J. Pharmacol. Exp. Ther., 251, 640 (1989).
Graham, R. M., Pettinger, W. A., Sagalowsky, A., Brabson, J., Gandler, T.: Renal alpha adrenergic receptor abnormality in the spontaneous hypertensive rat.Hypertension, 4, 881 (1982).
Pettinger, W. A., Sanchez, A., Saavedra, J., Haywood, J. R., Grandler Rodges, T.: Altered renal alpha2-adrenergic receptor regulation in genetically hypertensive rats.Hypertension, 4 (Suppl. 2), 188 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Neumann, P., Gröne, H.J. & Fuchs, E. Autoradiographic localization of prazosin and rauwolscine binding sites in the human kidney. International Urology and Nephrology 25, 19–28 (1993). https://doi.org/10.1007/BF02552250
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02552250