Skip to main content
SpringerLink
Log in

Cell cycle control of S phase: A comparison of two yeasts

  • Chromosoma Focus
  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

The mechanisms responsible for correct timing of DNA synthesis within the cell cycle and for limiting replication to one round per cell cycle are basically similar in the two model yeasts,Saccharomyces cerevisiae andSchizosaccharomyces pombe, despite many differences in detail. In both cases, the timing of initiation and the prevention of additional rounds are controlled by the activity levels of B-type cyclins. These similarities are likely to extend to other eukaryotic organisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Amon A, Irniger S, Nasmyth K (1994) Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until activation of G1 cyclins in the next cell cycle. Cell 77:1037–1050

    Article  PubMed  CAS  Google Scholar 

  • Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ (1996)SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86:263–274

    Article  PubMed  CAS  Google Scholar 

  • Blow JJ, Laskey RA (1988) A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332:546–548

    Article  PubMed  CAS  Google Scholar 

  • Broek D, Bartlett R, Crawford K, Nurse P (1991) Involvement of p34cdc2 in establishing the dependency of S phase on mitosis. Nature 349:388–393

    Article  PubMed  CAS  Google Scholar 

  • Chapman JW, Johnston LH (1989) The yeast gene,DBF4, essential for entry into S phase, is cell cycle regulated. Exp Cell Res 180: 419–428

    Article  PubMed  CAS  Google Scholar 

  • Cocker JH, Piatti S, Santocanale C, Nasmyth K, Diffley JFX (1996) An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature 379:180–182

    Article  PubMed  CAS  Google Scholar 

  • Correa-Bordes J, Nurse P (1995) p25ruml orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase. Cell 83:1001–1009

    Article  PubMed  CAS  Google Scholar 

  • Dahmann C, Diffley JFX, Nasmyth KA (1995) S-phase-promoting cyclin-dependent kinases prevent re-replication by inhibiting the transition of replication origins to a pre-replicative state. Current Biol 5:1257–1269

    Article  CAS  Google Scholar 

  • Diffley JFX (1995) The initiation of DNA replication in the budding yeast cell division cycle. Yeast 11: 1651–1670

    Article  PubMed  CAS  Google Scholar 

  • Diffley JFX, Cocker JH (1992) Protein-DNA interactions at a yeast replication origin. Nature 357: 169–172

    Article  PubMed  CAS  Google Scholar 

  • Diffley JFX, Cocker JH, Dowell SJ, Rowley A (1994) Two steps in the assembly of complexes at yeast replication origins in vivo. Cell 78:303–316

    Article  PubMed  CAS  Google Scholar 

  • Dirick L, Böhm T, Nasmyth K (1995) Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle ofSaccharomyces cerevisiae. EMBO J 14:4803–4813

    PubMed  CAS  Google Scholar 

  • Dowell SJ, Romanowski P, Diffley JFX (1994) Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo. Science 265:1243–1246

    Article  PubMed  CAS  Google Scholar 

  • Fenech M, Carr AM, Murray J, Watts FZ, Lehmann AR (1991) Cloning and characterisation of the rad4 gene ofSchizosaccharomyces pombe—a gene showing short regions of sequence similarity to the human xrcc1 gene. Nucl Acids Res 19:6737–6741

    PubMed  CAS  Google Scholar 

  • Fisher DL, Nurse P (1996) A single fission yeast mitotic cyclin B p34cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins. EMBO J 15:850–860

    PubMed  CAS  Google Scholar 

  • Hayles J, Fisher D, Woollard A, Nurse P (1994) Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex. Cell 78:813–822

    Article  PubMed  CAS  Google Scholar 

  • Heichman KA, Roberts JM (1996) The yeastCDC16 andCDC27 genes restrict DNA replication to once per cell cycle. Cell 85:39–48

    Article  PubMed  CAS  Google Scholar 

  • Hofmann JFX, Beach D (1994)cdtl is an essential target of the Cdc10/Sctl transcription factor: requirement for DNA replication and inhibition of mitosis. EMBO J 13:425–434

    PubMed  CAS  Google Scholar 

  • Irniger S, Piatti S, Michaelis C, Nasmyth K (1995) Genes involved in sister chromatid separation are needed for b-type cyclin proteolysis in budding yeast. Cell 81:269–277

    Article  PubMed  CAS  Google Scholar 

  • Jackson AL, Pahl PMB, Harrison K, Rosamond J, Sclafani RA (1993) Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein. Mol Cell Biol 13: 2899–2908

    PubMed  CAS  Google Scholar 

  • Jallepalli PV, Kelly TJ (1996) Rum1 and Cdc18 link inhibition of cyclin-dependent kinase to the initiation of DNA replication inSchizosacharomyces pombe. Genes Dev 10: 541–552

    PubMed  CAS  Google Scholar 

  • Kearsey SE, Maiorano D, Holmes EC, Todorov IT (1996) The role of MCM proteins in the cell cycle control of genome duplication. BioEssays 18:183–190

    Article  PubMed  CAS  Google Scholar 

  • Kelly TJ, Martin GS, Forsburg SL, Stephen RJ, Russo A, Nurse P (1993) The fission yeastcdc18 + gene product couples S phase to START and mitosis. Cell 74:371–382

    Article  PubMed  CAS  Google Scholar 

  • King RW, Peters J-M, Tugendreich S, Rolfe M, Hieter, P, Kirschner MW (1995) A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. Cell 81:279–288

    Article  PubMed  CAS  Google Scholar 

  • Maiorano D, Assendelft GBv, Kearsey SE (1996) Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle. EMBO J 15:861–872

    PubMed  CAS  Google Scholar 

  • Martín-Castellanos C, Labib K, Moreno S (1996) B-type cyclins regulate G1 progression in fission yeast in opposition to the p25ruml cdk inhibitor. EMBO J 15:839–849

    PubMed  Google Scholar 

  • Mondesert O, McGowan CH, Russell P (1996) Cig2, a B-type cyclin, promotes the onset of S inSchizosaccharomyces pombe. Mol Cell Biol 16: 1527–1533

    PubMed  CAS  Google Scholar 

  • Muzi-Falconi M, Brown GW, Kelly TJ (1996a)cdc18+ regulates initiation of DNA replication inSchizosaccharomyces pombe. Proc Natl Acad Sci USA 93:1566–1570

    Article  PubMed  CAS  Google Scholar 

  • Muzi-Falconi M, Brown GW, Kelly TJ (1996b) DNA replication: controlling initiation during the cell cycle. Curr Biol 6:229–233

    Article  PubMed  CAS  Google Scholar 

  • Nishitani H, Nurse P (1995) p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast. Cell 83:397–405

    Article  PubMed  CAS  Google Scholar 

  • Piatti S, Lengauer C, Nasmyth K (1995) Cdc6 is an unstable protein whosede novo synthesis in G1 is important for the onset of S phase and for preventing a ‘reductional’ anaphase in the budding yeastSaccharomyces cerevisiae. EMBO J 14:3788–3799

    PubMed  CAS  Google Scholar 

  • Piatti S, Böhm T, Cocker JH, Diffley JFX, Nasmyth K (1996) Activation of S-phase-promoting CDKs in late G1 defines a “point of no return” after which Cdc6 synthesis cannot promote DNA replication in yeast. Genes Dev 10:1516–1531

    PubMed  CAS  Google Scholar 

  • Saka Y, Yanagida M (1993) Fission yeastcu5 +, required for S phase onset and M phase restraint, is identical to the radiation-damage repair generad4 +. Cell 74:383–393

    Article  PubMed  CAS  Google Scholar 

  • Saka Y, Fantes P, Sutani T, McInerny C, Creanor J, Yanagida M (1994) Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. EMBO J 13:5319–5329

    PubMed  CAS  Google Scholar 

  • Schwob E, Bohm T, Mendenhall MD, Nasmyth K (1994) The B-type cyclin kinase inhibitor p40SICI controls the G1 to S transition inS. cerevisiae. Cell 79:233–244

    Article  PubMed  CAS  Google Scholar 

  • Schwob E, Nasymth K (1993)CLB5 andCLB6, a new pair of B cyclins involved in S phase and mitotic spindle formation inS. cerevisiae. Genes Dev 7:1160–1175

    PubMed  CAS  Google Scholar 

  • Singer JD, Manning BM, Formosa T (1996) Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism. Mol Cell Biol 16:1356–1366

    PubMed  CAS  Google Scholar 

  • Wuarin J, Nurse P (1996) Regulating S phase: CDKs, licensing and proteolysis. Cell 85:785–787

    Article  PubMed  CAS  Google Scholar 

  • Yoon H-J, Loo S, Campbell JL (1993) Regulation ofSaccharomyces cerevisiae CDC7 function during the cell cycle. Mol Biol Cell 4:195–208

    PubMed  CAS  Google Scholar 

  • Zachariae W, Nasmyth K (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7:791–801

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, 14263, Buffalo, NY, USA

    Joel A. Huberman

Authors
  1. Joel A. Huberman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huberman, J.A. Cell cycle control of S phase: A comparison of two yeasts. Chromosoma 105, 197–203 (1996). https://doi.org/10.1007/BF02528767

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02528767

Keywords

Search

Navigation