Abstract
Bone morphogenetic protein (BMP), associated with N,N-dicarboxymethyl chitosan, is used to induce or facilitate the repair of articular cartilage lesions. This association is intended for the synergistic potentiation of the respective biological effects. Data show that BMP-7 enhances the in vivo proliferation of cells with chondrocytes phenotype in the articular environment, leading to partial healing of the articular surface of the lesions. N,N-dicarboxymethyl chitosan is found to be useful as a molecular carrier or drug delivery agent.
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Albo, D., Long, C., Jhala, N., Atkinson, B., Granick, M. S., Wang, T., Meddahi, A., Barritault, D., andSolmon, M. P. (1996): ‘Modulation of cranial bone healing with heparin-like dextran derivatives’,J. Craniofac. Surg.,7, pp. 19–22
Ashina, I., Sampath, T. H., andHauschka, P. V. (1996): ‘Human osteogenic protein-1 induces chondroblastic, osteoblastic and/or adipocytic differentiation of clonal murine target cells’,Exp. Cell Res.,222, pp. 38–47
Bragi, V. M., Renkiewicz, R. R., Jordan, H., Bonadio, J., Hartman, J. W., andRoessler, B. J. (1995): ‘Transplantation of transduced chondrocytes protects articular cartilage from interleukin 1-induced extracellular matrix degradation’,J. Clin. Invest.,96, pp. 2454–2560
Brittberg, M., Lindahl, A., Nilsson, A., Ohlsson, C., Siögren-Jansson, E., andPeterson, L. (1995): ‘Autologous cartilage cell transplantation. The goal is pain relief and restored joint function’,Nord Med.,110, pp. 330–334
Buckwalter, J. A., Rosenberg, L. C., Coutts, R., Hunziker, E., Reddi, A. H., andMow, V. (1988): ‘Articular cartilage: injury and repair’,Woo, S. L., andBuckwalter, J. A. (Eds.): In ‘injury and repair of the muscoloskeletal soft-tissues’, (Am. Acad. Orthop-Surg., Park Ridge, IL) pp. 465–482
Chen, P., Vukievic, S., Sampath, T. K., andLuyten, F. P. (1993): ‘Bovine articular chondrocytes do not hypertophy when cultured in the presence of serum and osteogenic protein-1’,Biochem. Biophys. Res. Comm.,197, pp. 1253–1259
Ginsburg, G. T., Royster, D., Kassabian, G., Shuler, C. F., Dougherty, W. R., andSank, A. C. (1995): ‘Mesenchymal commitment to digital joint formation’,Ann. Plast. Surg.,19, pp. 95–104
Grgic, M., Jelic, M., Basic, V., Baic, N., Pecina, M., andVukicevic, S. (1997): ‘Regeneration of articular cartilage dfects in rabbit by osteogenic protein-1 (bone morphogenetic protein-7)’,Acta Med. Croatica.,51, pp. 23–27
Guerne, P. A., Blanco, F., Kaelin, A., Desgeorges, A., andLotz, M. (1995): ‘Growth factor responsiveness of human articular chondrocytes in ageing and development’,Arthritis Rheum.,38, pp. 960–968
Hunziker, E., andRosenberg, L. C. (1996): ‘Repair of partial-thickness defects in articular cartilage: cell recruitment from the synovial membrane’,J. Bone Joint Surg.,78, pp. 721–33
Ioffe, L. A., Liu, Y., andStanley, P. (1996): ‘Essential role for complex N-glycans in forming an organized layer of bronchial epithelium’,Proc. Natl. Acad. Sci. USA93, pp. 11041–11046
Israel, D. I., Nove, J., Kerns, K. M., Moutsatsos, I. K., andKaufman, R. J. (1992): ‘Expression and characterisation of bone morphogenetic protein-2 in Chinese hamster ovary cells’,Growth Factors,71, pp. 39–50
Iwata, H., Ono, S., Sato, K., Sato, T., andKawamura, M. (1993): ‘Bone morphogenetic protein-induced muscle- and synovium-derived cartilage differentiationin vitro’,Clin. Orthop.,296. pp. 295–300
Kapila, S., Lee, C., andRichard, D. W. (1995): ‘Characterisation and identification of proteinases and proteinase inhibitors synthesized by temporomandibular joint disc cells’,J. Dent. Res.74, pp. 1328–1336
Kato, Y., Nakashima, K., Sato, K., Yan, W., Iwamoto, M., andSuzuki, F. (1991): ‘Purification of Growth Factors from cartilage’,Meth. Enzym.198, pp. 416–424
Lietman, S. A., Yanagishita, M., Samath, T. K., andReddi, A. H. (1997): ‘Stimulation of proteoglycan synthesis in explants of porcine articular cartilage by recombinant osteogenic protein-1 (bone morphogenetic protein-7)’,J. Bone Joint Surg. Am.,79, pp. 1132–1137
Maleski, M. P., andKnudson, C. B. (1996): ‘Hyaluronan-mediated aggregation of limb bud mesenchyme and mesenchymal condensation during chondrogenesis’,Exp Cell Res.225, pp. 55–66
Matsuda, K., Nagasawa, N., Suzuki, S., Ishiki, N., andIkana, Y. (1995): ‘In vivo chondrogenesis in collagen sponge sandwiched by perichondrium’,J Biomater Sci. Polym. Ed.,7, pp. 221–229
Mattioli-Belmonte, M., Muzzarelli, B., andMuzzarelli, R. A. A. (1997): ‘Chitins and chitosans in wound healing and other biomedical applications’,Carbohydrates in Europe,19, pp. 30–36
Muehlman, C., andArsenis, Ch. (1995a): ‘Articular Cartilage part I: the normal joint’,J. Am. Podiatr. Med. Assoc.,85, pp. 277–281
Muehlman, C., andArsenis, Ch. (1995b): ‘Articular Cartilage part II: the osteoarthritic joint’,J. Am. Podiatr. Med. Assoc.,85, pp. 281–286
Muzzarelli, R. A. A., Bicchiega, V., Biagini, G., Pugnaloni, A., andRizzoli, C. (1992): ‘Role of the N-carboxybutylchitosan in the repair of the meniscus’,J. Bioact. Comp. Polym.,7, pp. 65–81
Muzzarelli, R. A. A., Ilari, P., andPetrarulo, M. (1994): ‘Solubility and structure of N-carboxymethyl chitosan’,Int. J. Biol. Macromol.,16, pp. 177–180
Muzzarelli, R. A. A. (Ed.) (1996): ‘Chitin Enzymology’, vols. 1 and 2. (ATEC, Grottammare (AP), Italy)
Muzzarelli, R. A. A. (1997): ‘Human enzymatic activities related to the therapeutic administration of chitin derivatives’,Cell Biol. Life Sci.,53, pp. 131–141
Muzzarelli, R. A. A., andTerbojevich, M. (1998): ‘6-Oxychitins, novel hyaluronan-like regiospecifically carboxilated chitins’, to be published inCharbohyd. Poly.
Muzzarelli, R. A. A., Ramos, V., Stanic, V., Dubini, B., Mattioli-Belmonte, M., Tosi, G., andGiardino, R.: ‘Osteogenesis promoted by calcium phosphate N,N-dicarboxymethyl chitosan’,Carbohydr. Pol., In press
Otani, K., Nita, I., Macauly, W., Georgescu, H. I., Robbins, P. D., andEvans, C. H. (1996): ‘Suppression of antigen-induced arthritis in rabbits byex vivo gene therapy’,J. Immunol.,156, pp. 3550–3562
Paralkar, V. M., Weeks, B. S., Yu, Y. M., Kleinmann, H. K., andReddi, A. H. (1992): ‘Recombinant human bone morphogenetic protein 2B stimulates PC12 cell differentiation: potentiation and binding to type IV collagen’J. Cell. Biol.,119, pp. 1721–1728
Rogers, J. J., Young, N. E., Adkison, L. R., Lucas, P. A., andBlack, A. C. Jr. (1995): ‘Differentiation factors induce expression of muscle, fat, cartilage and bone in a clone of mouse pluripotent mesenchymal stem cells’,Am. Surg.,61, pp. 231–236
Sams, A. E., andNixon A. J. (1995): ‘Chondrocyte-laden collagen scaffolds for resurfacing extensive articular cartilage defects’,Osteoarthritis Cartilage,3, pp. 47–59
Shigemasa, Y., andMinami, S. (1995): ‘Applications of chitin and chitosan for biomaterials’,Biotechnol. Genetic Eng. Rev.,13, pp. 383–420
Specchia, N., Gigante, A., Falciglia, F., andGreco, F. (1996): ‘Fetal chondral homograft in the repair of articular cartilage defects’,Bull. Hospital. Joint. Dis.,54, pp. 230–235
Trippel, S. B. (1995): ‘Growth factor actions on articular cartilage’,J. Rheumatol. Suppl.,43, pp. 129–132
Varki, A. (1996): ‘Does DG42 synthesise hyaluronan or chitin?’,Proc. Natl. Acad. Sci. USA,93, pp. 4523–4525
Wozney, J. M., Rosen, V., Celeste, A. J., Mitsock, L. M., Whitters, M. J., Kriz, R. W., Hewick, R. M., andWang, E. A. (1988): ‘Novel regulators of bone formation: Molecular clones and activities’,Science,242, pp. 1528–153
Yabu, M., Takaoka, K., Hashimoto, J., andFujita, H. (1992): ‘Immunohistochemical, autoradiographic and electron microscopic studies on transformation of fibroblasts in chondrocytes in the mouse subfascia induced by bone morphogenetic protein’,Histochem.,97, pp. 463–468
Young, H. E., Mancini, H. L., Wright, R. P., Smith, J. C., Black, A. C. Jr.,Regan, C. R., andLucas, P. A. (1995): ‘Mesenchymal stem cells reside within the connective tissues of many organs’,Dev. Dyn.,202, pp. 137–144
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Mattioli-Belmonte, M., Gigante, A., Muzzarelli, R.A.A. et al. N,N-dicarboxymethyl chitosan as delivery agent for bone morphogenetic protein in the repair of articular cartilage. Med. Biol. Eng. Comput. 37, 130–134 (1999). https://doi.org/10.1007/BF02513279
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DOI: https://doi.org/10.1007/BF02513279