Summary
Eight triazine herbicides, prometon, propazine, atrazine, simazine, prometryn, ametryn, metribuzin, and cyanazine, have been extracted from human whole blood and urine samples by headspace solid-phase microextraction (SPME) with a polydimethylsiloxane-coated fiber and quantified by capillary gas chromatography with nitrogen-phosphorus detection.
Extraction efficiencies for all compounds were 0.21–0.99% for whole blood, except for cyanazine (0.06%). For urine, the extraction efficiencies for prometon, propazine, atrazine, prometryn and ametryn were 13.6–38.1%, and those of simazine, metribuzin and cyanazine were 1.35–8.73%.
The regression equations for the compounds extracted from whole blood were linear within the concentration ranged 0.01–1 μg (0.5 mL)−1 for prometon, propazine, atrazine, prometryn, and ametryn, and 0.02–1 μg (0.5 mL)−1 for simazine, metribuzin, and cyanazine. For urine, regression equations for all compounds were linear within the concentration range 0.005–0.25 μg mL−1. Compound detection limits were 2.8–9.0 ng (0.5 mL)−1 and 0.4–2.0 ng mL−1 for whole blood and urine, respectively. The coefficients of within-day and day-to-day variation were satisfactory for all the compounds, and not greater than 10.3 and 14.2%, respectively.
Data obtained from determination of atrazine in rat whole blood after oral administration of the compound are also presented.
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Kumazawa, T., Lee, X.P., Kondo, K. et al. Determination of triazine herbicides in human body fluids by solid-phase microextraction and capillary gas chromatography. Chromatographia 52, 195–199 (2000). https://doi.org/10.1007/BF02490456
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DOI: https://doi.org/10.1007/BF02490456