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Expression of 60-kDa and inducible 70-kDa stress proteins in gentamicin-induced acute renal failure

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  • Experimental Investigations
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Abstract

Background

Heat shock proteins contribute to the survival of cells under stress conditions. We have previously observed the expression of HSP90 and constitutive HSP70 in rat kidneys during stress loading. This study was designed to determine whether HSP60 and HSP70i are induced in rat kidneys during exposure to gentamicin.

Methods

During the continuous administration of gentamicin (40 mg/kg per day) to rats for 30 days, the changes of HSP60 and HSP70i in these kidneys were quantitatively investigated by immunoblot analyses using specific antibodies.

Results

The indicators for renal failure,N-acetyl-β-d-glucosaminidase, blood urea nitrogen, and serum creatinine, increased, and reached the maximum levels at day 12. Thereafter, they spontaneously decreased, and were close to their normal levels at day 21, despite continued gentamicin exposure. The time course of HSP60 levels showed a unique 2-peak pattern exclusively in the cytosols of the cortex; the gentamicin target site. The second HSP60 peak occurred during the recovery phase, and coincided with published reports of a second peak of gentamicin accumulation in the kidney. The time course of HSP70i level showed a single peak at day 12 when the renal failure was most intense; the level was similar between the soluble and insoluble fractions in all portions of the kidney, except the inner medulla.

Conclusion

The increase in cytosolic HSP60 levels, as well as HSP70i levels, may be related to the spontaneous recovery from renal failure at the target site of the drug.

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Authors and Affiliations

  1. Second Department of Biochemistry, Akita University School of Medicine, 1-1-1 Hondo, 010-0000, Akita, Japan

    Guozhong Tao, Hideaki Itoh & Yohtalou Tashima

  2. Third Department of Internal Medicine, Akita University School of Medicine, 1-1-1 Hondo, 010-0000, Akita, Japan

    Atsushi Komatsuda & Akira B. Miura

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  1. Guozhong Tao
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  2. Atsushi Komatsuda
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  3. Akira B. Miura
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  4. Hideaki Itoh
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  5. Yohtalou Tashima
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Tao, G., Komatsuda, A., Miura, A.B. et al. Expression of 60-kDa and inducible 70-kDa stress proteins in gentamicin-induced acute renal failure. Clin Exper Neph 1, 254–260 (1997). https://doi.org/10.1007/BF02480637

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  • DOI: https://doi.org/10.1007/BF02480637

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