Abstract
In vivo andin situ perfusion techniques of the canine pancreas for physiologic and secretin response to studies are described. We developed these modified techniques to clarify the mechanism of the pancreatic exocrine secretion. An analysis was made of some physiological parameters of the pancreas and our findings were compared with those obtained in secretin stimulation of the pancreas. The composition of secretin induced venous effluent and pancreatic juice was investigated. A decrease of pH, pO2 and (HCO3 −) in the venous blood and a temporary efflux of K+ from the cell to the perfusate were observed after secretin stimulation. A temporary decrease of perfusion pressure was observed after secretin stimulation and the change is thought to be due to vascular dilatation. Ultrastructural findings from the pancreas perfused for about 1 hour showed no prominent changes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Augier, D., Boucard, J. P. et al.: Relationships between blood flow and secretion in the isolated perfused canine pancreas,J. Physiol., Lond. 221: 55–69, 1972.
Anune, S. and Semb, L. S.: The effect of secretin and pancreozymin on pancreatic blood flow in the conscious and anesthetized dog,Acta Physiol. Scand. 76: 406–414, 1969.
Babkin, F. P. and Starling, E. H.: A method for the study of the perfused pancreas,J. Physiol., Lond. 61: 245–249, 1926.
Case, R. M., Harper, A. A. and Scratchered, T.: Water and electrolyte secretion by the perfused pancreas of the cat,J. Physiol., Lond. 196: 133–149, 1967.
Ely, M. R., Kachelhoffer, J. et al.: Ex vivo vascular perfusion of the isolated canine pancreas,Eur. Surg. Res. 6: 341–353, 1974.
Kanno, T.: Perfusion of the pancreas,Seitai No Kagaku, 21: 487–496, 1970 (in Japanese).
Meyer, W., Castelfranchi, P. L. et al.: Physiologic Studies during perfusion of the isolated canine pancreas,Surg. Res. 5: 105–115, 1973.
Mohan Rao, M. and Elmslie, R. G.: A modified technique of isolated pancreatic perfusion,J. Surg. Res. 10: 357–362, 1970.
Nardi, G. L., Greep, J. K. et al.: Physiologic Peregrinations in Pancreatic perfusion,Ann. Surg. 158: 830–839, 1963.
Hermon-Taylor, J.: A technique for perfusion of the isolated canine pancreas,Grastroenterology 55: 488–501, 1968.
Ritchie, H. D., Hardcastle, J. D. et al.: Isolated organ perfusion for physiologic studies,Digestive Diseases 16: 81–87, 1971.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Teranishi, S., Okajima, K. Perfusion techniques of the canine pancreas for physiological and secretin response studies. The Japanese Journal of Surgery 9, 253–261 (1979). https://doi.org/10.1007/BF02469430
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02469430