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Identification of monoclonal antibody defined epitopes on human leukocyte antigens utilizing phage display peptide libraries

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Summary

Utilizing phage display peptide libraries, we have identified and mapped the antigenic determinants recognized by mouse monoclonal antibodies (mAb) on two sets of immunologically important molecules, HLA class I and class II antigens. Anti-HLA class I mAb TP25.99 recognizes a conformational and a linear determinant on distinct regions of the HLA class I α3 domain. Anti-HLA class I mAb HO-4 recognizes a conformational determinant on the α2 domain of HLA-A2 and A28 allospecificities. Anti-HLA-DR1,-DR4,-DR6,-DR8,-DR9 mAb SM/549 recognizes a conformational determinant on the β chain of HLA class II antigens. These results indicate the versatility of phage display peptide libraries to characterize antigenic determinants recognized by anti-HLA mAb.

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Authors and Affiliations

  1. Imclone Systems Inc., 10014, New York, NY, U.S.A.

    Qinwei Zhou

  2. Department of Microbiology and Immunology, New York Medical College, 10595, Valhalla, NY, U.S.A.

    Smruti A. Desai, Xinhui Wang, Elvyra J. Noronha, Mariangela Neri & Soldano Ferrone

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  1. Qinwei Zhou
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  2. Smruti A. Desai
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  3. Xinhui Wang
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  4. Elvyra J. Noronha
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  5. Mariangela Neri
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  6. Soldano Ferrone
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Zhou, Q., Desai, S.A., Wang, X. et al. Identification of monoclonal antibody defined epitopes on human leukocyte antigens utilizing phage display peptide libraries. Lett Pept Sci 6, 77–86 (1999). https://doi.org/10.1007/BF02443621

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  • DOI: https://doi.org/10.1007/BF02443621

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