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Identification of epitopes recognized by monoclonal antibodies directed against HTLV-I envelope surface glycoprotein using peptide phage display

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Abstract

Phage peptide libraries constitute powerful tools for themapping of epitopes recognized by monoclonal antibodies (mAbs).Using screening of phage displayed random peptide libraries wehave characterized the binding epitopes of three mAbs directedagainst the surface envelope glycoprotein (gp46) of the humanT-cell leukemia virus type I (HTLV-I). Two phage libraries,displaying random heptapeptides with or without flankingcysteine residues, were screened for binding to mAbs 7G5D8, DB4and 4F5F6. The SSSSTPL consensus sequence isolated fromconstrained heptapeptide library defines the epitope recognizedby DB4 mAb and corresponds to the exact region 249–252 of thevirus sequence. The APPMLPH consensus sequence isolated fromnon constrained heptapeptide library defines the epitoperecognized by 7G5D8 mAb and corresponds to the region 187–193with a single amino acid substitution, methionine to leucine atposition 190. The third consensus sequence LYWPHD isolated fromconstrained heptapeptide library defines the epitope recognizedby 4F5F6 mAb. It corresponds to an epitope without directequivalence with the virus sequence. The data presented hereshowed that 7G5D8 and DB4 mAbs are raised against linearepitopes while 4F5F6 mAb recognized a continuous topographic epitope.

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Author notes

  1. J. L. Chagnaud

    Present address: Diagast Laboratoires, 30 Avenue Canteranne, 33608, Pessac, France

Authors and Affiliations

  1. Laboratoire d'Immunologie Moléculaire et Parasitologie, Université Victor Segalen Bordeaux2, 146 rue Léo Saignat, 33076, Bordeaux, France

    J. L. Chagnaud, D. Moynet, J. H. Bezian & P. Vincendeau

  2. GISIR-ADERA, Centre Condorcet 162, Avenue A. Schweitzer, BP 196 33608, Pessac, France

    J. H. Bezian & B. Guillemain

  3. Laboratoire de Virologie, Université Victor Segalen Bordeaux2, 146 rue Léo Saignat, 33076, Bordeaux, France

    D. Londos-Gagliardi, H. Fleury & B. Guillemain

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  1. J. L. Chagnaud
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  2. D. Moynet
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  7. B. Guillemain
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Correspondence to D. Moynet.

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Chagnaud, J.L., Moynet, D., Londos-Gagliardi, D. et al. Identification of epitopes recognized by monoclonal antibodies directed against HTLV-I envelope surface glycoprotein using peptide phage display. Letters in Peptide Science 8, 95–106 (2001). https://doi.org/10.1023/A:1015093204280

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