Summary
Phage P1 does not adsorb toS. typhinurium wild type cells. It does adsorb to rough derivatives including strains with mutations in thegalE gene. Phage strain P1CMclr-100 can be efficiently propagated inS. typhimurium derivatives, either by induction of a lysogen, or by lytic infection.
Phage P1 lysates are able to mobilize genetic markers in a generalized fashion. The transduction system is essentially identical to that inEscherichia coli, except that CaCl2 is not required for efficient adsorption.
Two regions of theS. typhimurium chromosome were mapped by P1-mediated transduction. Several examples of genes linked by P1, and unlinked by P22, are presented. The relative efficiency of P1 over P22 in transduction was not determined, however.
Data presented indicate unambigously that the gene order for thetrp region is:his...dad A-hem A-trp-pyrF ...pyrC but known markers in between were not used. The gene order for thecys A region was determined to be as follows:phe A...pur C-cys A-trz A-pts-dsd-aro D-pur F...his, and special mapping problems for this region are discussed.
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Mojica-A, T. Transduction by phage P1CMclr-100 inSalmonella typhimurium . Molec. Gen. Genet. 138, 113–126 (1975). https://doi.org/10.1007/BF02428116
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DOI: https://doi.org/10.1007/BF02428116