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Neural morphological effects of long-term implantation of the self-sizing spiral cuff nerve electrode

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Abstract

The paper reports on the histological effects of chronic implantation of self-sizing spiral cuff nerve electrodes on the cat sciatic nerve. The implantation period is about 4.4 months. Four different experimental conditions are evaluated: control, sham, bare cuff (cuffs without contacts and leads) and full cuff. The total number of axons in the nerves of the control group is compared with the three other groups. The surface occupied by collagen fibres in the nerve section, perineurium thickness, fibre diameter and myelin thickness are also measured. The average number of axons in the control nerves is found to be 16416 (±1509) and does not differ significantly from the three other groups (p>0.1). Collagen measurements show an extrafascicular epineurial fibrosis in the two implanted groups that is found to be significantly different (p<0.05). No differences are encountered in the perineurium thickness analysis. Fibre diameter distributions show a regular bimodal pattern for all groups. Centrality (mean and Pm) and dispersion statistics (P25 and P75) extracted from fibre diameter distributions do not reveal significant differences. Myelin thickness distributions are also similar for all groups, as well as centrality and dispersion statistics. The present morphometrical results suggest that the effects produced by a chronic spiral cuff implant on this animal model are negligible.

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References

  • Agnew, W., McCreery, D., Yuen, T., andBullara, L. (1988): ‘Development of safe techniques for selective activation of neurons’. Technical Report, Contract N01-NS-62397, NIH-NINCDS

  • Agnew, W., McCreery, D., Yuen, T., andBullara, L. (1989): ‘Histological and physiological evaluation of electrically stimulated peripheral nerve: considerations for the selection of parameters’,Ann. Biomed. Eng.,17, pp. 39–60

    Google Scholar 

  • Agnew, W., andMcCreery, D. (1990): ‘Considerations for safety with chronically implanted nerve electrodes’,Epilepsia,31, (Suppl. 2), pp. S27-S32

    Google Scholar 

  • Aziz, W., Firrell, J., Ogden, L., andBreidenbach, W. (1999): ‘Blood flow in a chronic entrapment neuropathy model in the rabbit sciatic nerve’,J. Reconstr: Microsurg.,15, pp. 47–53

    Google Scholar 

  • Baer, G., Talonen, P., Shneerson, J., Markkula, H., Exner, G., andWells, F. (1990): ‘Phrenic nerve stimulation for central ventilatory failure with bipolar and four-pole electrode systems’,Pacing Clin. Electrophys.,13, pp. 1061–1072

    Google Scholar 

  • Bosnjak, R., Dolenc, V., andKralj, A. (1999): ‘Biomechanical response in the ankle stimulation of lumbosacral nerve roots with spiral cuff multielectrode: preliminary study’,Neurol. Med. Chirur.,39, pp. 659–666

    Google Scholar 

  • Brown, W., Ferguson, G. G., Jones, M., andYates, S. (1976): ‘The location of conduction abnormalities in human entrapment neuropathies’,Can. J. Neurol. Sci.,3, pp. 111–1118

    Google Scholar 

  • Chervin, R., andGuilleminault, C. (1997): ‘Diaphragm pacing for respiratory insufficiency’,J. Clin. Neurophysiol.,14, pp. 369–377

    Google Scholar 

  • Cornefjord, M., Sato, K., Olmarker, K., Rydevik, B., andNordborg, C. (1997): ‘A model for chronic root compression studies: Presentation of a porcine model for controlled, slow-onset compression with analyses of anatomic aspects, compression onset rate, and morphologic and neurophysiologic effects’,Spine,22, pp. 946–957

    Article  Google Scholar 

  • Creasey, G., Elefteriades, J., DiMarco, A., Talonen, P., Bijak, M., Girsch, W., andCantor, C. (1996): ‘Electrical stimulation to restore respiration’,J. Rehab. Res. Dev.,33, pp. 123–132

    Google Scholar 

  • Cruz-Orive, L., andWeibel, E. (1981): ‘Sampling designs for stereology’,J. Microscop. 122, pp. 235–257

    Google Scholar 

  • Dahlin, L., andKanje, M. (1992): ‘Conditioning effect induced by chronic nerve compression, an experimental study of the sciatic and tibial nerves of rats’,Seand. J. Reconstr. Hand Surg. 26, pp. 37–41

    Google Scholar 

  • Dahlin, L., Rydevick, B., McLean, W., andSjöstrand, J. (1984): ‘Changes in fast axonal transport during experimental nerve compression at low pressure’,Exp. Neurol.,84, pp. 29–36

    Article  Google Scholar 

  • Dyck, P. (1969): ‘Experimental hypertrophy neuropathy’,Arch. Neurol.,21, pp. 73–82

    Google Scholar 

  • Dyck, P., Thomas, P., Lambert, E., andBunge, R. (1984): ‘Peripheral neuropathy’,in W.B. Saunders Company, pp. 769, 802

  • Fern, R., andHarrison, P. (1994): ‘The contribution of ischaemia and deformation to the conduction block generated by compression of the cat sciatic nerve’,Exp. Physiol.,79(4), pp. 583–592

    Google Scholar 

  • Glenn, W., andPhelps, M. (1985): ‘Diaphragm pacing by electrical stimulation of the phrenic nerve’,Neurosurgery,17, pp. 974–984

    Google Scholar 

  • Grill, W., andMortimer, J. (1994): ‘Electrical properties of implant encapsulation tissue’,Ann. Biomed. Eng.,22, pp. 22–33

    Google Scholar 

  • Gundersen, H. (1977): ‘Notes on the estimation of the numerical density of arbitrary profiles: the edge effects’,J. Microsc.,111, pp. 219–223

    Google Scholar 

  • Heijmans, H. (1999): ‘Connected morphological operators for binary images’,Comput. Vis. Image Underst.,73(1), pp. 99–120

    MATH  MathSciNet  Google Scholar 

  • Kim, J., Manuelidis, E., William, W., Glenn, W., Fukuda, Y., Cole, D., andHogan, J. (1983): ‘Light and electron microscopic studies of phrenic nerves after long-term electrical stimulation’,J. Neurosurg.,58, pp. 84–91

    Google Scholar 

  • Larsen, J. (1998): ‘Stereology of nerve cross sections’,J. Neurosci. Methods,85, pp. 107–118

    Article  Google Scholar 

  • Larsen, J., Thomsen, M., Haugland, M., andSinkjaer, T. (1998): ‘Degeneration and regeneration in rabbit peripheral nerve with long-term nerve cuff electrode implant: a stereological study of myelinated and unmyelinated axons’,Acta Neuropathol.,96, pp. 365–378

    Article  Google Scholar 

  • Lequesne, P., andCasey, E. (1974): ‘Recovery of conduction velocity distal to a compressive lesion’,J. Neurol. Neurisurg. Psychiatry,37, pp. 1346–1351

    Google Scholar 

  • Lloyd, D., andChang, H. (1948): ‘Afferent fibers in muscle nerves’,J. Neurophysiol.,11, pp. 199

    Google Scholar 

  • Lundborg, G. (1970): ‘Experimental studies on intraneural microvascular pathophysiology and nerve function in a limb subjected to a temporary circulatory arrest’,Scand. J. Plast. Reconstr. Surg.,6(Suppl.), pp. 287–294

    Google Scholar 

  • Lundborg, G. (1975): ‘Structure and function of the intraneural microvessels as related to trauma, edema formation and nerve function’,J. Bone Joint Surg.,57A, pp. 938–948

    Google Scholar 

  • Mackinnon, S., Dellon, A., andHudson, A. (1984): ‘Chronic nerve compresion: an experimental model in the rat’,Ann. Plast. Surg.,13, pp. 112–120

    Google Scholar 

  • Mackinnon, S., Dellon, A., andHudson, A. (1985): ‘A primate model for chronic nerve compression’,J Reconstr. Microsurg.,1, pp. 185–195

    Google Scholar 

  • Mayhew, T., andSharma, A. (1984a): ‘Sampling schemes for estimating nerve fiber size. i. methods for nerve trunks of mixed fascicularity’,J. Anat.,139, pp. 45–58

    Google Scholar 

  • Mayhew, T., andSharma, A. (1984b): ‘Sampling schemes for estimating nerve fiber size. ii. methods for unifascicular nerve trunks’,J. Anat.,139, pp. 59–66

    Google Scholar 

  • McCreery, D., Agnew, W., Yuen, T., andBullara, L. (1992): ‘Damage in peripheral nerve from continuous electrical stimulation: comparison of two stimulus waveforms’,Med. Biol. Eng. Comput.,30, pp. 109–114

    Google Scholar 

  • McCreery, D., Yuen, T., Agnew, W., andBullara, L. (1994): ‘Stimulus parameters affecting tissue injury during microstimulation in the cochlear nucleus of the cat’,Hear. Res.,77(1–2), pp. 105–115

    Google Scholar 

  • McNeal, D., Waters, R., andReswick, J. (1977): ‘Experience with implanted electrodes’,Neurosurg.,1, pp. 228–229

    Google Scholar 

  • Mortimer, J. (1990): ‘Electrode for functional neuromuscular stimulation’. Technical Report, Contract N01-NS-7-2396, final report, NIH-NINCDS Neural prostheses program

  • Naples, G., Mortimer, J., Schneider, A., andSweeney, J. (1988): ‘A spiral nerve cuff electrode for peripheral nerve stimulation’,IEEE Trans.,BME-35, pp. 905–916

    Google Scholar 

  • Nielson, K., Watts, C., andClark, W. (1976): ‘Peripheral nerve injury from implantation of chronic stimulating electrodes for pain control’,Surg. Neurol.,5, pp. 51–53

    Google Scholar 

  • Ochoa, J., Danta, G., Fowler, G., andGilliatt, R. (1971): ‘Nature of the nerve lesion caused by pneumatic torniquet’,Nature,233, pp. 265–271

    Article  Google Scholar 

  • Romero, E., Cuisenaire, O., Denef, J., Delbeke, J., Macq, B., andVeraart, C. (2000): ‘Automatic morphometry of nerve histological sections’,J. Neurosci. Methods,97, pp. 111–122

    Article  Google Scholar 

  • Rydevik, B., andLundborg, G. (1977): ‘Permeability of intraneural microvessels and perineurium following acute, graded experimental nerve compression’,Scand. J. Plast. Reconstr: Surg.,11, pp. 179–187

    Google Scholar 

  • Rydevik, B., Lundborg, G., andBagge, U. (1981): ‘Effects of graded compression on intraneural blood flow’,J. Hand Surg.,6, pp. 3–12

    Google Scholar 

  • Sauter, J., Berthord, H., andJeanrenaud, B. (1983): ‘Simple electrode for intact nerve stimulation and/or recording in semichronic rats’,Pflugers Arch.,397, pp. 68–69

    Article  Google Scholar 

  • Segal, R., Stacey, B., Rudy, T., Baser, S., andMarkham, J. (1998): ‘Spinal cord stimulation revisited’,Neurol. Res.,20, pp. 391–396

    Google Scholar 

  • Strauss, W., andSprague, J. (1944): ‘The dinnervation of the interosseous muscles and the mechanism of the toe spreading reflex of the hind-limb in the cat’,Am. J. Physiol.,142, pp. 391–396

    Google Scholar 

  • Suderland, S. (1968): ‘Nerves and nerve injuries’ in E. and S. Livingstone

  • Upshaw, B., andSinkjaer, T. (1997): ‘Natural versus artifitial sensors applied in peroneal nerve stimulation’,J. Appl. Physiol.,82, pp. 227–231

    Google Scholar 

  • Van Kerrebroeck, E., Der Aa, H.V., Bosch, J., Koldewijn, E., andVorsteveld, J. (1997): ‘Sacial rhizotomies and electrical bladder stimulation in spinal cord injury. Part i: Clinical and urodynamic analysis. dutch study group on sacral anterior root stimulation’,Eur. Urol.,31, pp. 263–271

    Google Scholar 

  • Veraart, C., Grill, W., andMortimer, J. (1993): ‘Selective control of muscle activation with a multipolar nerve cuff electrode’,IEEE Trans.,BME-40, pp. 640–653

    Google Scholar 

  • Weese-Mayer, D., Morrow, A., Brouillette, R., Ilbawi, M., andHunt, C. (1989): ‘Diphragm pacing in infants and children. a lifetable analysis of implanted components’,Am. Rev. Resp. Dis.,139, pp. 974–979

    Google Scholar 

  • Weisl, H., andOsborne, G. (1964): ‘The pathological changes in rats' nerves subject to moderate compression’,J. Bone Joint Surg.,46B, pp. 297–306

    Google Scholar 

  • Yuen, T., Agnew, W. andBullara, L. (1984): ‘Histopathological evaluation of dog sacral nerve after chronic electric stimulation for micturition’,Neurosurg.,14, pp. 449–455

    Google Scholar 

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Authors and Affiliations

  1. Neural Rehabilitation Engineering Laboratory, Université Catholique de Louvain, Brussels, Belgium

    E. Romero, J. Delbeke &  C. Veraart

  2. Histology Laboratory, Université Catholique de Louvain, Brussels, Belgium

    J. F. Denef

  3. Public Health School, Université Catholique de Louvain, Brussels, Belgium

    A. Robert

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  1. E. Romero
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  2. J. F. Denef
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  3. J. Delbeke
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  4. A. Robert
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  5. C. Veraart
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Correspondence to C. Veraart.

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Romero, E., Denef, J.F., Delbeke, J. et al. Neural morphological effects of long-term implantation of the self-sizing spiral cuff nerve electrode. Med. Biol. Eng. Comput. 39, 90–100 (2001). https://doi.org/10.1007/BF02345271

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  • DOI: https://doi.org/10.1007/BF02345271

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