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Chromatographic behaviour and purification of N1.5-glycosyl enkephalins

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Summary

N 1.5-Glycosyl enkephalins are potent analgesics. Two enkephalin-related glycosyl peptides: [DMet2, Pro5] enkephalin (N 1.5 β-glucopyranosyl] amide and [DMet2, Pro5] enkephalin [N 1.5 β-galactosyl] amide have been prepared following the liquid phase procedure. The chromatographic behavior of both the final glycosyl enkephalins and their synthetic intermediates on reversed-phase columns has been tested. Semipreparative, reversed-phase, HPLC conditions have been developed in order to obtain a simple and rapid procedure for the purification of the two synthetic pentapeptides. Thus, using isocratic elution, injections of 10mg of crude material after gel filtration gave homogeneous (99.9%) glycosyl enkephalins.

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Authors and Affiliations

  1. Peptide Research Section, Dep. of Biological Organic Chemistry, C.I.D., C.S.I.C., c/Jordi Girona, 18-26, E-08034, Barcelona, Spain

    J. L. Torres, P. Clapés, I. Haro, G. Valencia, F. Reig & J. M. García Antón

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  1. J. L. Torres
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  2. P. Clapés
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  3. I. Haro
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  4. G. Valencia
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  5. F. Reig
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  6. J. M. García Antón
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Torres, J.L., Clapés, P., Haro, I. et al. Chromatographic behaviour and purification of N1.5-glycosyl enkephalins. Chromatographia 25, 891–894 (1988). https://doi.org/10.1007/BF02311424

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  • DOI: https://doi.org/10.1007/BF02311424

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