Abstract
Rice (Oryza sativa L.) plantlets regenerated from callus (rice regenerants) were grownin vitro during the preparation stage either on a 1/4 strength N6 gellan gum (4 g l-1) medium without sucrose (SFM) or with 30 g l-1 sucrose (SCM), and under CO2 concentrations of 0.4, 2, 10, 50 or 100 mmol mol-1, a photoperiod of 24 h and a photosynthetic photon flux density (PPFD) of 125 μmol m-2 s-1. Rice regenerants were also grownin vitro on SFM or SCM under CO2 concentration of 50 mmol mol-1, a photoperiod of 12 or 24 h and a PPFD of 80 or 125 μmol m-2 s-1. All rice regenerants grew successfully on SFM under CO2 concentrations of 50 or 100 mmol mol-1. Increasing the CO2 concentration increased the survival percentage, shoot length and shoot and root dry weights of rice regenerants grown on SFM. Increasing CO2 concentration had no significant effect on the survival or growth of rice regenerants grown on SCM. Survival percentages of rice regenerants grown on SCM were less than 80% for each of the CO2 concentrations. A photoperiod of 24 h under CO2 enrichment improved the survival and growth of rice regenerants grown on SFM, and increased the survival percentage and shoot dry weight of rice regenerants grown on SCM.
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Abbreviations
- CH:
-
casein acid hydrolysate
- MES:
-
2-(N-morpholino) ethanesulfonic acid
- NAA:
-
naphthaleneacetic acid
- N6:
-
Chu et al. (1975) basal medium composition
- PPFD:
-
photosynthetic photon flux density
- SFM:
-
sugar-free medium
- SCM:
-
sugar-containing medium
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
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Seko, Y., Nishimura, M. Effect of CO2 and light on survival and growth of rice regenerants grownin vitro on sugar-free medium. Plant Cell Tiss Organ Cult 46, 257–264 (1996). https://doi.org/10.1007/BF02307103
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DOI: https://doi.org/10.1007/BF02307103