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Highly efficient separations on 5 μm internal diameter open tubular capillaries coated with thick polyacrylate stationary phases

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Summary

Fused silica capillaries with an internal diameter of 5 μm were coated with polyacrylate films containing either alkyl or ether functionalities. Polymer networks were created byin situ photopolymerization of acrylic monomers. Capillaries with phase ratios up to 1.3, having excellent separation efficiencies, could be prepared. The performance of these capillaries was demonstrated by the separation of various solute mixtures. Nineteen phenylthiohydantoin (PTH) amino acid derivatives could be separated in less then 10 minutes when using UV detection. Laser Induced Fluorescence detection was used for the separation of polycyclic aromatic hydrocarbons, dansyl (Dns) derivatized amino acids ando-phthalaldehyde (OPA) derivatized aliphatic amines which could be separated with approximately 310,000 plates in less than 3.5 minutes corresponding to around 1500 plates per second.

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Authors and Affiliations

  1. Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, The Netherlands

    R. Swart, J. C. Kraak & H. Poppe

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  1. R. Swart
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  2. J. C. Kraak
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  3. H. Poppe
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Swart, R., Kraak, J.C. & Poppe, H. Highly efficient separations on 5 μm internal diameter open tubular capillaries coated with thick polyacrylate stationary phases. Chromatographia 40, 587–593 (1995). https://doi.org/10.1007/BF02290273

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  • DOI: https://doi.org/10.1007/BF02290273

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