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HPLC separation and fluorimetric estimation of chlorophylls and pheophytins in fresh and frozen peas

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Summary

A HPLC method for the separation and estimation of chlorophylls and pheophytins in fresh and frozen peas was assessed with the aim of following the colour changes of the products during storage under frozen conditions.

The method involves the acetone extraction of the pigments, followed by HPLC on RP18 with isocratic elution by acetone: ethanol:water, (70:17:13), detecting and estimating the separated compounds by fluorimetry. The mobile phase composition was selected by monitoring the separation efficiency of several mixtures of solvents by HPLC. The reliability and the accuracy of the method were checked.

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Authors and Affiliations

  1. I.V.T.P.A.-Istituto Sperimentale per la Valorizzazione Tecnologica dei Prodotti Agricoli, Via G. Venezian n. 26, I-20133, Milano, Italy

    E. Forni, M. Ghezzi & A. Polesello

Authors
  1. E. Forni
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  2. M. Ghezzi
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  3. A. Polesello
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Forni, E., Ghezzi, M. & Polesello, A. HPLC separation and fluorimetric estimation of chlorophylls and pheophytins in fresh and frozen peas. Chromatographia 26, 120–124 (1988). https://doi.org/10.1007/BF02268135

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  • DOI: https://doi.org/10.1007/BF02268135

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