Summary
Adenosine mono-, di- and triphosphates, inosine monophosphates, adenosine, inosine and hypoxanthine were separated in a single run by high-performance liquid chromatography with UV detection. AC-18 reversephase column was used. An isocratic elution with 100mM phosphate buffer (KH2PO4) pH6.5 and 1.2% methanol, followed by a 5 minute linear gradient up to 15% methanol after the 10th minute was performed during each run with a flow rate of 1.25ml/min. The time required for each analysis was 25 minutes. The detection limit for each substance ranged from 3 to 5 pmol. All the substances under study were detected in the incubation medium when adenosine triphosphate was exogenously applied to the innervated frog sartorius muscle.
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Cunha, R.A., Sebastião, A.M. & Ribeiro, J.A. Separation of adenosine triphosphate and its degradation products in innervated muscle of the frog by reverse phase high-performance liquid chromatography. Chromatographia 28, 610–612 (1989). https://doi.org/10.1007/BF02260687
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DOI: https://doi.org/10.1007/BF02260687