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Some recent advances in thin-layer chromatography

II. Application of direct spectrophotometry and direct fluorometry in amino acid, peptide and nucleic acid chemistry

Fortschritte in der Dünnschicht-Chromatographie

II. Spektrophotometrische und fluorimetrische Direkt-Messung in der Aminosäure-, Peptid- und Nucleinsäure-Chemie

Quelques progrès récents en chromatographie sur couche mince

II. Application de la spectrophotométrie et de la fluorométrie directes dans la chime des amino-acides, peptides, et acides nucléiques

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Summary

Spectrophotometric and fluorometric direct evaluation of thin-layer chromatograms is described. Scanning can be carried out by determination of spectral reflectance, fluorescence and fluorescence quenching. The techniques described are suitable for characterization and identification of microgram amounts of substances by directly plotting their reflectance or fluorescence spectra. Several examples (amino acids, DNP-, DANS-, PTH-amino-acids, nucleic acid derivatives) are given, influencing factors and the reproducibility are discussed.

Zusammenfassung

Die spectrophotometrische und fluorometrische Direktauswertung von Dünnschicht-Chromatogrammen wird beschrieben. Quantitative Auswertungen können durchgeführt werden durch Messung der Remission, Fluoreszenz und der Fluoreszenzlöschung. Die beschriebenen Techniken eignen sich zur Charakterisierung und Identifizierung von Mikrogramm-Mengen von Substanzen, durch direkte Aufnahme ihrer Remissions- oder Fluoreszenzspektren. Zahlreiche Beispiele (Aminosäuren, DNP-, DANS-, PTH-Aminosäuren, Nukleinsäure-Derivate) werden angegeben, Einflußfaktoren und die Reproduzierbarkeit werden diskutiert.

Sommaire

On décrit l'évaluation directe, par spectrophotométrie et par fluorométrie, de chromatogrammes sur couche mince. Cette évaluation peut se faire par détermination soit de la réflexion spectrale, soit de la fluorescence, soit de l'extinction de la fluorescence. Les techniques décrites se prètent à la caractérisation et identification de substances, dans la région des microgrammes, par enregistrement direct de leurs spectres de reflexion ou de fluorescence. Plusieurs exemples (amino-acides, DNP-, DANS-, PTH-amino-acides, dérivés d'acides nucléiques) sont donnés, et sont discutés les différents paramètres ainsi que la reproductibilité.

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References

  1. Pataki, G., Borko, J., Curtius, H. Ch. andTanc, Chromatographia1, 406 (1968)

    Google Scholar 

  2. Pataki, G. andWang, K. T., J. Chromatogr., in press.

  3. Pataki, G., Dünnschichtchromatographie in der Aminosäure-und Peptid chemie, W. de Gruyter, Berlin 1966

    Google Scholar 

  4. Pataki, G., Techniques of Thin-Layer Chromatography in Amino Acid and Peptide Chemistry, Ann Arbor Science Publ., Ann Arbor 1968

    Google Scholar 

  5. Pataki, G., Chromatogr. Rev.9, 23 (1967)

    Google Scholar 

  6. Randerath, K. andRanderath, E., in Methods of Enzymology (eds.L. Grossman andK. Moldave)12, part A, 323 (1967)

  7. Pataki, G., Advances in Chromatography (eds.C. Giddings andR. A. Keller) 7, in press (1968)

  8. Geiss, F., J. Chromatogr.33, 9, 108 (1968) and references cited therein

    Google Scholar 

  9. Decker, P., Naturwissenschaften45, 464 (1958)

    Google Scholar 

  10. Brenner, M., Pataki, G. andNiederwieser, A., in Thin-Layer Chromatography (editor G.B. Marini-Bettolo), Elsevier Amsterdam 1964, p. 116

    Google Scholar 

  11. Brenner, M., in Stationary Phase in Paper- and Thin-Layer Chromatography (eds.:K. Macek andI. M. Hais), Elsevier, Amsterdam, 1965, p. 322

    Google Scholar 

  12. Snyder, L. R., Advances in Chromatography (eds.:C. Giddings andR. A. Keller,4, 3 (1967)

    Google Scholar 

  13. Snyder, L. R., Principles of Adsorption Chromatography, Marcel Dekker, New York, 1968

    Google Scholar 

  14. Dallas, M. S. J., J. Chromatogr.33, 58 (1968)

    Google Scholar 

  15. Galanos, D. S. andKapoulas, V. M., J. Chromatogr.13, 128 (1964), cf. also reference [3]Pataki, G., Dünnschichtchromatographie in der Aminosäure-und Peptid chemie, W. de Gruyter, Berlin 1966, p. 56 and reference [4]Pataki, G., Techniques of Thin-Layer Chromatography in Amino Acid and Peptide Chemistry, Ann Arbor Science Publ., Ann Arbor 1968, p. 47

    Google Scholar 

  16. Williams, D. A., J. Chromatogr.26, 280 (1967)

    Google Scholar 

  17. Pataki, G., Borko, J. andKunz, A., Z. Klin, Chem. Biochem, in press

  18. Randerath, K., andRanderath, E., J. Chromatogr.22, 110 (1966)

    Google Scholar 

  19. Pataki, G., 4th Symp. on Chromatography and Electrophoresis, Brussels 1966, p. 305

  20. Kaiser, R., in Dünnschichtchromatographie (editor:E. Stahl), Springer, Berlin 1967, p. 114

    Google Scholar 

  21. Frodyma, M. M., Frei, R. W. andWilliams, D. J., J. Chromatogr.13, 61 (1964)

    Google Scholar 

  22. Frodyma, M. M. andFrei, R. W., J. Chromatogr.15, 501 (1964)

    Google Scholar 

  23. Frodyma, M. M., andFrei, R. W., J. Chromatogr.17, 131 (1965)

    Google Scholar 

  24. Frei, R. W., andFrodyma, M. M., Analyt. Biochem.9, 310 (1964)

    Google Scholar 

  25. Frei, R. W., andFrodyma, M. M., Analyt. Chim. Acta32, 501 (1965)

    Google Scholar 

  26. Frei, R. W., andZeitlin, H., Analyt. Chim. Acta32, 32 (1965)

    Google Scholar 

  27. Frodyma, M. M., Lieu, V. T., andFrei, R. W., J. Chromatogr.18, 520 (1965)

    Google Scholar 

  28. Frei, R. W., Fukui, I. T., Lieu, V. T., andFrodyma, M. M., Chimia (Aarau)20, 23 (1966)

    Google Scholar 

  29. Frodyma, M. M., andLieu, V. T., Analyt. Chem.39, 814 (1967)

    Google Scholar 

  30. Frei, R. W., Ryan, D. E., andLieu, V. T., Canad. J. Chem.44, 1945 (1966)

    Google Scholar 

  31. Frei, R. W., andRyan, D. E., Analyt. Chim. Acta37, 187 (1967)

    Google Scholar 

  32. Lieu, V. T., Frodyma, M. M., Higashi, L. S., andKunimoto, L. H., Anal. Biochem.19, 454 (1967)

    Google Scholar 

  33. Zaye, D. F., Frei, R. W., andFrodyma, M. M., Anal. Chim. Acta39, 13 (1967)

    Google Scholar 

  34. Frodyma, M. M., Zaye, D. F., andLieu, V. T., Anal. Chim. Acta40, 451 (1968)

    Google Scholar 

  35. Frei, R. W., Liiva, R., andRyan, D. E., Canad. J. Chem.46, 167 (1968)

    Google Scholar 

  36. Frei, R. W., andNomura, N. S., Mikrochimica Acta1968, 565

  37. Kortüm, G., andVogel, J., Angew. Chemie71, 451 (1959)

    Google Scholar 

  38. Kortüm, G., Braun, W., andHerzog, G., Angew. Chemie75, 653 (1963)

    Google Scholar 

  39. Braun, W., andKortüm, G., Zeiss Informationen16, 27 (1968)

    Google Scholar 

  40. Frei, R. W., Zeitlin, H., andFrodyma, M. M., Chemische Rundschau (Solothurn, Switzerland)19, Nr. 13 (1966), p. 41

    Google Scholar 

  41. De Galan, L., Van Leeuwen, J., andCamstra, K., Anal. Chim. Acta35, 395 (1966)

    Google Scholar 

  42. Klaus, R., J. Chromatogr.16, 311 (1964)

    Google Scholar 

  43. Klaus, R., Pharm. Ztg.112, 480 (1967)

    Google Scholar 

  44. Huber, W., J. Chromatogr.33, 378, (1968)

    Google Scholar 

  45. Pamphlet 50-657/K-d and 50-657/K-c, Zeiss, Oberkochen (Germany)

  46. Jork, H., 3rd Intern. Chromatogr. Symp., Brussels 1964, p. 295

  47. Jork, H., 4th Intern. Chromatogr. Symp., Brussels 1966, p. 227

  48. Jork, H., Z. anal. Chem.221, 17 (1966)

    Google Scholar 

  49. Jork, H., Cosmo Pharma3, 33 (1967)

    Google Scholar 

  50. Jork, H., Cosmo Pharma4, 12 (1968)

    Google Scholar 

  51. Stahl, E., andJork, H., Zeiss Information16, 52 (1968)

    Google Scholar 

  52. Stahl, E., Z. anal. Chem.236, 294 (1968)

    Google Scholar 

  53. Jork, H., Z. anal. Chem.236, 310 (1968)

    Google Scholar 

  54. Jork, H., J. Chromatogr.33, 297 (1968)

    Google Scholar 

  55. Struck, H., Karg, H. andJork, H., J. Chromatogr.,36, 74 (1968)

    Google Scholar 

  56. Jork, H., in Quantitative Paper and Thin-Layer Chromatography, Academic Press, London, in press

  57. Jork, H., Habilitationsschrift, University Saarbrücken, Germany, to be published

  58. Jork, H., Lecture on 120th Dechema Colloguiun, Frankfurt (Germany) 1965

  59. Pataki, G., unpublished experiments

  60. Moffat, E. D., andLyttle, R. L., Anal. Chem.31, 926 (1959)

    Google Scholar 

  61. Seiler, N., Werner, G., andWiechmann, M., Naturwissenschaften50, 643 (1963)

    Google Scholar 

  62. Seiler, N., andWiechmann, M., Z. analyt. Chemie220, 109 (1966)

    Google Scholar 

  63. Seiler, N., Z. physiol. Chem.348, 765 (1967)

    Google Scholar 

  64. Sawicki, E., andJohnson, H., Mikrochimica Acta1964, 435

  65. Sawicki, E., andJohnson, H., Microchemical J.8, 85 (1964)

    Google Scholar 

  66. Sawicki, E., Stanley, T. W., andElbert, W. C., Occupat. Health Review16, 8 (1964)

    Google Scholar 

  67. Sawicki, E., Stanley, T. W., Pfaff, J. D., andElbert, W. C., Analyt. Chim. Acta31, 359 (1964)

    Google Scholar 

  68. Sawicki, E., Stanley, R. W., Analyt. Chem.37, 938 (1965)

    Google Scholar 

  69. Bender, D. F., Sawicki, E., andWilson, R. M., Int. J. Air Water Pollution8, 633 (1964)

    Google Scholar 

  70. Sawicki, E., Stanley, T. W., Elbert, W. C., andMorgan, M., Talanta12, 605 (1965)

    Google Scholar 

  71. Sawicki, E., Johnson, H., andMorgan, M., Mikrochimica Acta1967, 297

  72. Sawicki, E., Stanley, T. W., andElbert, W. C., J. Chromatogr.20, 348 (1965)

    Google Scholar 

  73. Sawicki, E., andJohnson, H., J. Chromatogr.23, 142 (1966)

    Google Scholar 

  74. Pamphlet Q TL-65, Gerät zur quantitativen Direktauswertung von Dünnschichtchromatogrammen mit Hilfe der Fluorometrie; Pamphlet FT-67, Fluorometrie, Camag, Muttenz (BL), Switzerland.

  75. Jänchen, D., 4th Sympl. on Chromatogr. Electr., Brussels 1966, p. 194

  76. Jänchen, D., andPataki, G., J. Chromatogr.33, 391 (1968)

    Google Scholar 

  77. Pataki, G., andStrasky, E., Chimia (Aarau)20, 361 (1966)

    Google Scholar 

  78. Pataki, G., andWang, K. T., unpublished experiments.

  79. Ptaki, G., andKunz, A., J. Chromatogr.23, 465 (1966)

    Google Scholar 

  80. Pataki, G., andStrasky, E., 4th Intern. Symp. Chromatogr. Electroph., Brussels 1966, p. 317

  81. Pataki, G., andNiederwieser, A., J. Chromatogr.29, 133 (1967)

    Google Scholar 

  82. Pataki, G., Advances in Chromatography (C. Giddings andR. A. Keller, editors), Vol. 7 in press (1968)

  83. Brenner, M., Niederwieser, A., Pataki, G., andWeber, R., in Dünnschichtchromatographic (E. Stahl), Springer, Berlin, 1962, p. 79

    Google Scholar 

  84. Brenner, M., Niederwieser, A., Pataki, G., andWeber, R., in Thin-Layer Chromatography (E. Stahl), Springer, Berlin 1965, p. 75.

    Google Scholar 

  85. Niederwieser, A., andBrenner, M., Experientia21, 50, 105 (1965)

    Google Scholar 

  86. Niederwieser, A., andHonegger, C. G., Advances in Chromatography (C. Giddings andR. A. Keller)2, 123 (1966)

    Google Scholar 

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Authors and Affiliations

  1. Analytical Department, Laboratory for Chromatography, Robapharm LTD., Basle, Switzerland

    G. Pataki

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  1. G. Pataki
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Additional information

11th communication on “Application of thin-layer chromatography in sequence analysis of peptides”; 10th communication cf. reference [1]; 8th communication on “Quantitative thinlayer chromatography; 7th communication cf. reference [2]; 6th communication on “Thin-layer chromatography of nucleic acid bases, nucleosides, nucleotides and related compounds”; 5th communication cf. reference [1].

Part of a lecture given in March 1968 at St. Mary's University, Halifax N.S.; St. Dunstans University Charlottetown, P.E.I., Dalhousie University, Halifax, N.S.; Mt. Allison University, Sackville, N.B.; University of New Brunswick, Frederickton, N.B.; Acadja. University, Wolfsville, N.S. (Canada); Columbia University and Rockefeller University, New York, cf.G. Pataki andE. Strasky, Lecture on the 5th International Symposium on the Chemistry of Natural Products, London 8–13 July 1968, Abstracts p. 27.

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Pataki, G. Some recent advances in thin-layer chromatography. Chromatographia 1, 492–503 (1968). https://doi.org/10.1007/BF02255770

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