Abstract
Colletotrichum trifolii is a fungal pathogen which is responsible for anthracnose disease of alfalfa. To initiate research on molecular communication in this fungus, a kinase-encoding gene (TB3) and the corresponding cDNA were cloned and sequenced. The deduced amino acid sequence ofTB3 closely resembles that of aNeurospora crassa serine/threonine protein kinase, COT1, required for hyphal elongation and branching. The C-terminal catalytic domains of TB3 and COT1 are highly conserved but the N-terminal regions are divergent, particularly in the homopolymeric glutamine repeats of TB3. Northern analysis indicated thatTB3 expression was highest 1 h after inducing conidial germination and 1 h before germ tubes were first observed. Expression of TB3 transcripts returned to constitutive levels by 4 h after induction of germination.TB3 complemented thecot-1 mutant ofNeurospora crassa, demonstrating the functional conservation of this kinase between a pathogenic and a saprophytic fungus.
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Communicated by E. Cerdá-Olmedo
This manuscript has been assigned Journal Series No. 10931, Agricultural Research Division, University of Nebraska
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Buhr, T.L., Truesdell, G.M., Huang, C. et al. A kinase-encoding gene fromColletotrichum trifolii complements a colonial growth mutant ofNeurospora crassa . Molec. Gen. Genet. 251, 565–572 (1996). https://doi.org/10.1007/BF02173646
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DOI: https://doi.org/10.1007/BF02173646