Abstract
TheSaccharomyces cerevisiae geneABC1 is required for the correct functioning of thebc 1 complex of the mitochondrial respiratory chain. By functional complementation of aS. cerevisiae abc1 − mutant, we have cloned aSchizosaccharomyces pombe cDNA, whose predicted product is 50% identical to the Abc1 protein. Significant homology is also observed with bacterial, nematode, and even human amino acid sequences of unknown function, suggesting that the Abc1 protein is conserved through evolution. The cloned cDNA corresponds to a singleS. pombe geneabc1Sp, located on chromosome II, expression of which is not regulated by the carbon source. Inactivation of theabc1Sp gene by homologous gene replacement causes a respiratory deficiency which is efficiently rescued by the expression of theS. cerevisiae ABC1 gene. The inactivated strain shows a drastic decrease in thebc 1 complex activity, a decrease in cytochromeaa3 and a slow growth phenotype. To our knowledge, this is the first example of the inactivation of a respiratory gene inS. pombe. Our results highlight the fact thatS. pombe growth is highly dependent upon respiration, and thatS. pombe could represent a valuable model for studying nucleo-mitochondrial interactions in higher eukaryotes.
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Communicated by D. M. Lonsdale
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Bonnefoy, N., Kermorgant, M., Dujardin, G. et al. Cloning by functional complementation, and inactivation, of theSchizosaccharomyces pombe homologue of theSaccharomyces cerevisiae geneABC1 . Molec. Gen. Genet. 251, 204–210 (1996). https://doi.org/10.1007/BF02172919
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DOI: https://doi.org/10.1007/BF02172919