Skip to main content
SpringerLink
Log in

Platelets and polymorphonuclear leukocytes in experimental ocular inflammation in the rabbit eye

  • Laboratory Investigations
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Accumulation of111indium-oxine (111In)-labelled platelets and the kinetics of111In-labelled polymorphonuclear leukocytes (PMNLs) were studied in the anterior eye during neurogenic inflammation (induced by topical neutral formaldehyde) or after paracentesis in the rabbit, after formaldehyde irritation, 40 times more platelets were found in the aqueous humor 60 min later than in the control eyes and 400 times more after paracentesis. Platelets were found to be increased in the ciliary body, but not in the iris or choroid. Under light microscopy, some of the blood vessels in the ciliary processes were occluded and filled with red blood cells and platelets. The amount of PMNLs increased in the aqueous humor 15–20 h following formaldehyde irritation. After paracentesis, increased amounts of PMNLs were found in the iris and ciliary body 3–6 h later, while in the aqueous humor PMNLs were observed already 2 h. In the present study, increased amounts of111In-labelled platelets and PMNLs were demonstrated in the anterior eye during experimental ocular inflammation. This method provides a useful tool for evaluating the accumulation of cells or the cell kinetics in ocular tissues during experimental inflammation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ben-Zvi A, Rodrigues MM, Gery I, Schiffmann E (1981) Induction of ocular inflammation by synthetic mediators. Arch Ophthalmol 99:1436–1444

    Google Scholar 

  2. Bhattacherjee F, Hammond B, Salmon JA, Stepney R, Eakins KE (1981) Chemotactic response to some arachidonic acid lipoxygenase products in the rabbit eye. Eur J Pharmacol 73:21–28

    Google Scholar 

  3. Bonne C, Latour E, Driot JY, Coquelet C (1988) The participation of the platelet in blood-aqueous barrier breakdown induced by paracentesis in the rabbit eye. Proc Int Soc Eye Res V:33

    Google Scholar 

  4. Butler JM, Hammond By (1977) Effect of sensory denervation on the response of the rabbit eye to bradykinin and prostaglandin E1. Trans Ophthalmol Soc UK 97:668–674

    Google Scholar 

  5. Butler JM, Hammond BR (1980) The effects of sensory denervation on the responses of the rabbit eye to prostaglandin E1, bradykinin and substance P. Br J Pharmacol 69:495–502

    Google Scholar 

  6. Butler JM, Unger WG, Hammond BR (1979) Sensory mediation of the ocular response to neutral formaldehyde. Exp Eye Res 28:577–589

    Google Scholar 

  7. Camras CB, Bito LZ (1980) The pathophysiological effects of nitrogen mustard on the rabbit eye. I. The biphasic intraocular pressure response and the role of prostaglandins. Exp Eye Res 30: 41–52

    Google Scholar 

  8. Cole DF, Unger WG (1973) Prostaglandins as mediators for the responses of the eye to trauma. Exp Eye Res 17:357–368

    Google Scholar 

  9. Eakins KE (1977) Prostaglandin and non-prostaglandin mediated breakdown of the blood-aqueous barrier. Exp Eye Res [Suppl] 25:483–498

    Google Scholar 

  10. Goetzl EJ, Sun FF (1979) Generation of unigue mono-hydroxy-eicosatetraenoic acids from arachidonic acid by human neutrophils. J Exp Med 150:406–411

    Google Scholar 

  11. Howes EL, Cole PW, Cruse VK, Pollycove M (1988) Quantitation of acute experimental ocular inflammation with 111-indium leukocytes. Invest Ophthalmol Vis Sci 29:429–436

    Google Scholar 

  12. Issekutz AC, Movat HZ (1980) The in vivo quantitation and kinetics of rabbit neutrophil leukocyte accumulation in the skin in response to chemotactic agents andEscherichia coli. Lab Invest 42:310–317

    Google Scholar 

  13. Jampol LM, Neufeld AH, Sears ML (1975) Pathways for the response of the eye to injury. Invest Ophthalmol 14:184–189

    Google Scholar 

  14. Klein EM, Bito LZ (1983) Species variations in the pathophysiological responses of vertebrate eyes to a chemical irritant, nitrogen mustard. Invest Ophthalmol 24:184–191

    Google Scholar 

  15. Krootila K, Uusitalo H, Lehtosalo JI, Palkama A (1986) Effect of topical chemical irritation on the blood-aqueous barrier of the rat eye. Ophthalmic Res 18:248–252

    Google Scholar 

  16. Krootila K, Uusitalo H, Lehtosalo JI, Palkama A (1987) Recovery of the blood-aqueous barrier after topical chemical irritation in the rabbit eye. Graefe's Arch Clin Exp Ophthalmol 225:272–276

    Google Scholar 

  17. Krootila K, Uusitalo H, Palkama A (1988) Effect of neurogenic irritation and calcitonin gene-related peptide (CGRP) on ocular blood flow in the rabbit. Curr Eye Res 7:695–703

    Google Scholar 

  18. Leibowitz HM, Lass JH, Kupferman A (1974) Quantitation of inflammation in the cornea. Arch Ophthalmol 92:427–430

    Google Scholar 

  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  20. Neufeld AH, Jampol LM, Sears ML (1972) Aspirin prevents the disruption of the blood-aqueous barrier in the rabbit eye. Nature 238:158–159

    Google Scholar 

  21. Piotrowski W, Foreman JC (1986) Some effects of calcitonin gene-related peptide in human skin and on histamine release. Br J Dermatol 114:37–46

    Google Scholar 

  22. Scheffel U, McIntyre PA, Evatt B, Dvornicky JA, Natarajan TK, Boiling DR, Murphy EA (1977) Evaluation of indium-111 as a new high photon yield gamma-emitting “physiological” platelet label. Johns Hopkins Med J 140:285–293

    Google Scholar 

  23. Thakur ML, Segal AW, Louis L, Welch MJ, Hopkins J, Peters TJ (1977) Indium-111-labeled cellular blood components: mechanism and intracellular location in human neutrophils. J Nucl Med 18:1020–1024

    Google Scholar 

  24. Unger WG, Cole DF, Hammond BR (1975) Disruption of the blood-aqueous barrier following paracentesis in the rabbit. Exp Eye Res 20:255–270

    Google Scholar 

  25. Unger WG, Terenghi G, Ghatei MA, Ennis KW, Butler JM, Zhang SQ, Too HP, Plak JM, Bloom SR (1985) Calcitonin gene-related polypeptide as a mediator of the neurogenic ocular injury response. J Ocular Pharmacol 1:189–199

    Google Scholar 

  26. Uusitalo H (1984) The effect of autonomic receptor blockers on the ocular response to topical chemical irritation. Acta Physiol Scand 121:1–8

    Google Scholar 

  27. Williams RN, Paterson CA (1984) PMN accumulation in aqueous humour and iris-ciliary body during intraocular inflammation. Invest Ophthalmol 25:105–108

    Google Scholar 

  28. Williams RN, Paterson CA, Eakins EK, Bhattacherjee P (1983) Quantification of ocular inflammation: evaluation of polymorphonuclear leukocyte infiltration by measuring myeloperoxidase activity. Curr Eye Res 2:465–470

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, Eye Research Laboratory, University of Helsinki, SF-00170, Helsinki, Finland

    Kari Krootila, Juha I. Lehtosalo & Hannu Uusitalo

  2. Blood Transfusion Service, Finnish Red Cross, Helsinki, Finland

    Martti Syrjälä

Authors
  1. Kari Krootila
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Martti Syrjälä
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Juha I. Lehtosalo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hannu Uusitalo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krootila, K., Syrjälä, M., Lehtosalo, J.I. et al. Platelets and polymorphonuclear leukocytes in experimental ocular inflammation in the rabbit eye. Graefe's Arch Clin Exp Ophthalmol 227, 465–469 (1989). https://doi.org/10.1007/BF02172900

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02172900

Keywords

Search

Navigation