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Supression of growth ofVibrio cholerae in the intestine of immunized mice

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Abstract

In ligated intestinal loops of actively immunized adult mice, growth ofV. cholerae 569B was suppressed approximately seven fold when compared to bacterial growth in non-immune animals. Similarly, growth ofV. choleare 569B was reduced in mice immunized with a hybrid vibrio strain, NCV569B-165, which shares only flagella antigens withV. cholerae 569B. Immunofluorescence studies of intestinal loop contents and intestinal sections indicated that, in non-immune mice, vibrios coated the intestinal mucosa. In contrast, the intestinal mucosa of immune animals was almost free of bacteria. The vibrios were found agglutinated in the intestinal lumen contents of immune animals. It was concluded that immunization suppressed the growth ofV. cholerae in the intestinal lumen and the possibility that bacterial agglutination mediates this growth suppression is discussed.

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Author notes

  1. J. E. C. Bellamy (Fellow of the Medical Research Council of Canada)

    Present address: Department of Veterinary Pathology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

Authors and Affiliations

  1. Department of Microbiology, University of Adelaide, Adelaide, Australia

    Jürgen Knop & J. E. C. Bellamy (Fellow of the Medical Research Council of Canada)

  2. Hautklinik der Westfälischen Wilhelms-Universität, v.-Esmarchstr. 56, D-4400, Münster, Federal Republic of Germany

    Jürgen Knop

Authors
  1. Jürgen Knop
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  2. J. E. C. Bellamy
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Knop, J., Bellamy, J.E.C. Supression of growth ofVibrio cholerae in the intestine of immunized mice. Med Microbiol Immunol 162, 153–158 (1976). https://doi.org/10.1007/BF02121325

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  • DOI: https://doi.org/10.1007/BF02121325

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