Summary
3-Aminopyridine adenine dinucleotide phosphate (AADP) was prepared from NADP and 3-aminopyridine through the pig brain NADase-catalyzed pyridine base exchange reaction. The purified dinucleotide was chemically characterized and spectral properties of the compound were determined. The importance of the application of AADP in studies of NADP-requiring biochemical processes was indicated by the demonstration of AADP as an effective inhibitor of five NADP-requiring enzymes, by the demonstration of the fluorescence enhancement on the binding of AADP to yeast glucose-6-phosphate dehydrogenase when glucose-6-phosphate is present, and by the functioning of AADP as a fluorimetric substrate for snake venom nucleotide pyrophosphatase.
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Abbreviations
- AAD:
-
3-aminopyridine adenine dinucleotide
- AADP:
-
3-aminopyridine adenine dinucleotide phosphate
References
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This work was supported by Research Grant GB 25652 from the National Science Foundation.
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Anderson, B.M., Yuan, J.H. & Vercellotti, S.V. Studies of 3-Aminopyridine adenine dinucleotide phosphate. Mol Cell Biochem 8, 89–96 (1975). https://doi.org/10.1007/BF02116237
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DOI: https://doi.org/10.1007/BF02116237