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Parvalbumin-immunoreactive proteins in the nervous system of planarians

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Summary

1. Using immunological methods, we have identified parvalbumin-like material in the triclade flatworms,Polycelis nigra, Polycelis auriculata, Crenobia alpina, Dugesia tahitiensis, andDugesia polychroa.

2. Western immunoblot analysis of these five species revealed heat stable parvalbumin-immunoreactive proteins between 32 kD and 44 kD.

3. Proteins at 19 and 32 kD revealed intense labeling with45Ca2+.

4. Double immunodiffusion of planarian supernatants showed complete fusion of precipitates, indicating immunological relatedness of the parvalbuminlike material among the species investigated.

5. Immunocytochemical studies exhibit parvalbumin-immunoreactive material exclusively in neurons supporting the notion of an early evolutionary appearance of these proteins in the nervous system.

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Authors and Affiliations

  1. Department of Animal-Physiology, University of Salzburg, Institute of Zoology, Hellbrunnerstr. 34, A-5020, Salzburg, Austria

    A. Hutticher, H. H. Kerschbaum, V. Kainz, M. Bito & A. Hermann

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  1. A. Hutticher
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  2. H. H. Kerschbaum
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  3. V. Kainz
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  4. M. Bito
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  5. A. Hermann
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Hutticher, A., Kerschbaum, H.H., Kainz, V. et al. Parvalbumin-immunoreactive proteins in the nervous system of planarians. Cell Mol Neurobiol 15, 401–410 (1995). https://doi.org/10.1007/BF02071876

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  • DOI: https://doi.org/10.1007/BF02071876

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