Abstract
Optimal conditions for coupling sheep anti-T3 IgG to a solid phase are presented. We found that the optimal activation of microcrystalline cellulose was achieved with 0.15M 1,1′-carbonyldiimidazol (CDI) in acetone. We also found that using a 25 mg/cm3 anti-T3 IgG solution, in barbitone buffer 0.05M, pH 8.0, we could get a reasonable yield of coupling and a remaining solution of anti-T3 IgG (first supernatant) with a suitable concentration (≊10 mg/cm3) for another coupling. The solid phase anti-T3 obtained in these two couplings present similar characteristics which make possible their use in a total T3 RIA.
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References
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Santos, I., Waerenborgh, F. & Patricio, L. Coupling of a sheep anti-T3 IgG to a solid phase for a triiodothyronine radioimmunoassay. Journal of Radioanalytical and Nuclear Chemistry, Articles 102, 143–148 (1986). https://doi.org/10.1007/BF02037955
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DOI: https://doi.org/10.1007/BF02037955