Skip to main content
SpringerLink
Log in

Quantification of secretin release by acid, using immunocytochemistry and radioimmunoassay

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Zusammenfassung

Die Reaktion auf das Einträufeln einer 0,1N HCl (11 ml/min) in das Duodenum des Schweines wurde parallel mit dem Radioimmuntest zur Bestimmung des Sekretingehaltes der Mucosa und mit quantitativer immunhistochemischer Analyse der duodenalen Sekretionzellen untersucht. Nach einer 30minütigen Einwirkung der Säure betrug der durchschnittliche Abfall des zellulären Sekretingehaltes in 5 Schweinen 52%. Der entsprechende Wert, der mit der Radioimmuntestmethode ermittelt wurde, betrug 72%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. J. E. Jorpes andV. Mutt, Acta chem. scand.15, 1790 (1961).

    Google Scholar 

  2. V. Mutt andJ. E. Jorpes,Secretin: Isolation and determination of structure (abst.) (Proc. I. U. P. A. 4th Int. Congr. on the Chemistry of Natural Products, Stockholm June/July 1966, Sect. 2C-3).

  3. M. Bodanszky, M. A. Ondetti, S. D. Levine, V. L. Narayanan, M. von Saltza, J. T. Sheehan, N. J. Williams andE. F. Sabo, Chemy Ind.42, 1757 (1966).

    Google Scholar 

  4. M. Bodanszky andN. J. Williams, J. Am. chem. Soc.89, 6753 (1967).

    Article  PubMed  Google Scholar 

  5. G. Bussolati, C. Capella, E. Solcia, G. Vassallo andP. Vezzadini, Histochemie21, 1 (1971).

    Article  Google Scholar 

  6. J. M. Polak, S. R. Bloom, I. Coulling andA. G. E. Pearse, Gut12, 605 (1971).

    Google Scholar 

  7. J. M. Polak, S. R. Bloom, I. Coulling andA. G. E. Pearse, Scand. J. Gastroenterol.6, 739 (1971).

    PubMed  Google Scholar 

  8. S. R. Bloom andO. Ogawa, J. Endocr.58, 24 (1973).

    Google Scholar 

  9. S. R. Bloom, S. Joffe, Julia M. Polak andA. G. E. Pearse, to be published.

  10. A. G. E. Pearse, Julia M. Polak, Caroline Adams andP. A. Kendale, Histochem. J.6, 347 (1974).

    Article  PubMed  Google Scholar 

  11. J. H. Meyer, L. W. Way andM. I. Grossman, Am. J. Physiol.219, 971 (1970).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Histochemistry, Surgery and Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, W12 OHS, London, (England)

    Julia M. Polak, A. G. E. Pearse, S. Joffe & S. R. Bloom

Authors
  1. Julia M. Polak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. G. E. Pearse
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Joffe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. R. Bloom
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

We are grateful to ProfessorsViktor Mutt andJohn Brown for the provision of secretin, motilin and anti-Gip serum. We thank MrC. Lorden and MrD. Chappell for the design and construction of the tissue punch, MrF. Paice for Image Analysis, MissAviva Petrie for statistical help and Miss M-V.McCrossan for skilled technical assistance. The work was carried out with the aid of a joint M. R. C. Grant.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Polak, J.M., Pearse, A.G.E., Joffe, S. et al. Quantification of secretin release by acid, using immunocytochemistry and radioimmunoassay. Experientia 31, 462–464 (1975). https://doi.org/10.1007/BF02026380

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02026380

Keywords

Search

Navigation