Abstract
In intact adult dogs of both sexes exogenous growth hormone induced a marked increase in new bone formation. The net effect on resorption could not be defined quantitatively but endosteal resorption was decreased. Intracortical resorption was increased but intracortical porosity rose only slightly. In addition to the decrease in endosteal resorption, there was abundant endosteal new bone formation. This rise in endosteal new bone formation plus the marked stimulus to periosteal new bone formation led to anet increase in skeletal mass. The bone formed was normal histologically and microradiographically. Despite a marked rise in new bone formation, alkaline phosphatase values remained unchanged.
Résumé
Chez des chiens adultes, des deux sexes, l'injection d'hormone de croissance induit une augmentation marquée d'os nouvellment formé. L'effet sur la résorption n'a pu être définie quantitativement, mais la résorption au niveaude de l'endoste est augmentée. Il en est de même de la résorption intracorticale, mais la porosité intracorticale n'augmente que légèrement. En plus de la décroissance de la résorption de l'endoste, on observe du même côté une nette augmentation de la formation osseuse. Cette apposition osseuse ajoutée à la formation d'os périosté donne uneaugmentation de la masse squelettique. L'os formé est histologiquement et microradiographiquement normal. Malgré une nette augmentation de la formation osseuse, les valeurs de la phosphatase alcaline restent inchangées.
Zusammenfassung
Exogenes Wachstumshormon rief bei intakten ausgewachsenen Hunden beiden Geschlechts eine starke Zunahme der Knochenbildung hervor. Die resultierende Wirkung auf die Resorption konnte quantitativ nicht festgestellt werden, jedoch war die endostale Resorption vermindert. Die intracorticale Resorption war erhöht, aber die intracorticale Porosität stieg nur wenig an. Neben der Verminderung der endostalen Resorption war reichliche endostale Knochenneubildung festzustellen. Dieser Anstieg der endostalen Knochenneubildung plus die starke Anregung der periostalen Knochenneubildung führten zu einertatsächlichen Zunahme der Skelettmasse. Der gebildete Knochen war histologisch und mikroradiographisch normal. Trotz der starken Zunahme der Knochenneubildung blieben die Werte der alkalischen Phosphatase unverändert.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bartter, F. C.: Treatment of osteoporosis by calcium infusions. Proc. roy. Soc. Med.63, 339–340 (1970).
Bauer, G. C. H.: Tracer techniques for study of bone metabolism in man. Advanc. biol. Med. Phys.10, 227–275 (1965).
Daughaday, W. H., Kipnis, D. M.: The growth-promoting and anti-insulin action of somatotropin. Recent Progr. Hormone Res.22, 49–99 (1966).
Detenbeck, L. S., Jowsey, J.: Normal aging in the bone of the adult dog. Clin. Orthop.65, 76–80 (1969).
Dymling, J. F.: Calcium kinetics in osteopenia and parathyroid disease. Acta med. scand.175, (Suppl. 408), 1–56 (1964).
Fraser, R., Harrison, M., Ibbertson, K.: Rate of calcium turnover in bone. Quart. J. Med.24, 85–111 (1960).
Frost, H. M.: Bone dynamics in metabolic bone disease. J. Bone Jt Surg. A48, 1192–1203 (1966).
Frost, H. M.: Osteoporosis: Disturbance in osteoclasia. J. Amer. Geriat. Soc.9, 1078–1085 (1961).
Garn, S. M., Rohmann, C. G., Wagner, B.: Bone loss as general phenomenon in man. Fed. Proc.26, 1729–1736 (1967).
Harris, W. H., Haywood, E. A., Hamblen, D. L., Lavorgna, J.: Analysis of covariance of bone formation rates by specific skeletal sites and over time, p. 415–422. In “Les Tissus Calcifies”, Ve Symposium Européen, ed. by G. Milhaud, M. Owen and H. J. J. Blackwood, Paris: Societé D'Edition D'Enseignement Superieure 1968.
Harris, W. H., Haywood, E. A., Lavorgna, J., Hamblen, D. L.: Spatial and temporal variations in cortical bone formation in dogs: long-term study. J. Bone Jt Surg. A50, 1118–1128 (1968).
Harris, W. H., Heaney, R. P.: Skeletal renewal and metabolic bone disease. New Engl. J. Med.280, 193–202, 253–259, 303–311 (1969).
Harris, W. H., Weinberg, E. H.: Microscopic method of measuring increases in cortical bone volume and mass. Calc. Tiss. Res.8, 190–196 (1972).
Heins, J. N., Garland, J. T., Daughaday, W. H.: Incorporation of35S-sulfate into rat cartilage explantsin vitro: Effects of aging on responsiveness to stimulation by sulfation factor. Endocrinology87, 688–692 (1970).
Iskrant, A. P., Smith, R. W., Jr.: Osteoporosis in women 45 years and over related to subsequent fractures. Pub. Hth Rep. (Wash.)84, 33–38 (1969).
Jee, W. S. S., Bromley, R. G., Dockum, N. L., Lowe, M., Burrggraal, R., Dedekind, K., Arnold J. S.: Studies of trabecular bone in beagles and humans, p. 99–121. In Research in Radiobiology. Radiobiology Division of the Department of Anatomy, University of Utah College of Medicine 1965.
Jowsey, J., Hoye, R. C., Pak, C. Y. C., Bartter, F. C.: The treatment of osteoporosis with calcium infusions. Evaluation of bone biopsies. Amer. J. Med.47, 17–22 (1969).
Jowsey, J., Kelly, P. J., Riggs, B. L., Bianco, A. J., Scholz, D. A., Gershon-Cohen, J.: Quantitative microradiographic studies of normal and osteoporotic bone. J. Bone Jt Surg. A47, 785–806 (1965).
Pak, C. Y. C., Zisman, E., Evens, R., Jowsey, J., Delea, C. S., Bartter, F. C.: The treatment of osteoporosis with calcium infusions. Clinical studies. Amer. J. Med.47, 7–16 (1969).
Ray, R. D., Mueller, K. H., Sankaran, B., Mensen, E. D., Schwartz, T. B.: Metabolic diseases of bone: Kinetic studies. Med. Clin. N. Amer.49, 241–258 (1965).
Riggs, B. L., Jowsey, J., Kelly, P. J., Jones, J. D., Mahler, F. T.: Effect of sex hormones on bone in primary osteoporosis. J. clin. Invest.48, 1065–72 (1969).
Root, A. W., Oski, F. A.: Effects of human growth hormone in elderly males. J. Geront.24, 97–104 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harris, W.H., Heaney, R.P., Jowsey, J. et al. Growth hormone: the effect on skeletal renewal in the adult dog I. Morphometric studies. Calc. Tis Res. 10, 1–13 (1972). https://doi.org/10.1007/BF02012530
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02012530