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Enterobacter cloacae cross-colonization in neonates demonstrated by ribotyping

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Abstract

The intestinal colonization byEnterobacter cloacae strains with a derepressed cephalosporinase was studied in a paediatric ward between February 1990 and January 1991. Environmental sampling was performed simultaneously. Fifty-two isolates were recovered from 200 neonates (stool, blood) and 14 strains were isolated from the neonatal environment. An epidemiological study based on the typing of 36Enterobacter cloacae isolates was carried out using antibiotyping, biotyping and ribotyping methods. The isolates selected were from 21 neonates (24 isolates), the neonatal ward environment (8 isolates) and from other wards (4 isolates). Thirty-two isolates had the same antibiotic resistance pattern, corresponding to a derepressed cephalosporinase and resistance to the following aminoglycosides: kanamycin, gentamicin, tobramycin and netilmicin. No predominant biotyping pattern could be established. Ribotyping done with two endonucleases (EcoRI andBamHI) showed 28Enterobacter cloacae isolates to have a single pattern. Ribotyping was the most discriminating method used in this study, permitting identification of cross-contamination withEnterobacter cloacae in the paediatric ward.

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References

  1. Spritzer R, Kamp H, Dzoljic G, Sauer P: Five years of cefotaxime use in a neonatal intensive care unit. Pediatric Infectious Disease Journal 1990, 9: 92–96.

    Article  PubMed  CAS  Google Scholar 

  2. Bryan CS, John JF Jr, Pai MS, Austin TL: Gentamicin versus cefotaxime for therapy of neonatal sepsis. American Journal of Diseases of Children 1985, 139: 1086–1089.

    PubMed  CAS  Google Scholar 

  3. Andersen BM, Sorlie D, Hotvedt R: Multiply beta-lactam resistantEnterobacter cloacae infections linked to the environmental flora in a unit of cardiothoracic and vascular surgery. Scandinavian Journal of Infectious Diseases 1989, 21: 181–191.

    PubMed  CAS  Google Scholar 

  4. Flynn DM, Weinstein RA, Nathan C, Gaston MA, Kabins SA: Patients' endogenous flora as the source of “nosocomial”Enterobacter in cardiac surgery. Journal of Infectious Diseases 1987, 156: 363–368.

    PubMed  CAS  Google Scholar 

  5. Johnson MP, Ramphal R: Beta-lactam-resistantEnterobacter bacteriemia in febrile neutropenic patients receiving monotherapy. Journal of Infectious Diseases 1990, 162: 981–983.

    PubMed  CAS  Google Scholar 

  6. McConkey SJ, Coleman DC, Falkiner FR, McCann SR, Daly PA:Enterobacter cloacae in a haematology/oncology ward. First impressions. Journal of Hospital Infection 1989, 14: 277–284.

    Article  PubMed  CAS  Google Scholar 

  7. Modi N, Damjanovic V, Cooke RWI: Outbreak of cephalosporin resistantEnterobacter cloacae infection in a neonatal intensive care unit. Archives of Disease in Childhood 1987, 62: 148–151.

    Article  PubMed  CAS  Google Scholar 

  8. Gaston MA:Enterobacter, an emerging nosocomial pathogen. Journal of Hospital Infection 1988, 11: 197–208.

    Article  PubMed  CAS  Google Scholar 

  9. Gaston MA, Bucher C, Pitt TL: O Serotyping scheme forEnterobacter cloacae. Journal of Clinical Microbiology 1983, 18: 1079–1083.

    PubMed  CAS  Google Scholar 

  10. Gaston MA: Isolation and selection of a bacteriophage-typing set forEnterobacter cloacae. Journal of Medical Microbiology 1987, 24: 285–290.

    Article  PubMed  CAS  Google Scholar 

  11. Daw MA, Corcoran GB, Falkiner FR, Keane CT: Application and assessment of cloacin typing ofEnterobacter cloacae. Journal of Hospital Infection 1992, 20: 141–151.

    Article  PubMed  CAS  Google Scholar 

  12. Wang CC, Chu ML, Ho LJ, Hwang RC: Analysis of plasmid pattern in paediatric intensive care unit outbreaks of nosocomial infection due toEnterobacter cloacae. Journal of Hospital Infection 1991, 19: 33–40.

    Article  PubMed  CAS  Google Scholar 

  13. Richard C: Une méthode simple de marquage épidémiologique: la biotypie, application àEnterobacter cloacae etEscherichia coli. Bulletin de l'Association des Anciens Élèves de l'Institut Pasteur 1981, 87: 14–21.

    Google Scholar 

  14. Grimont F, Grimont PAD: Ribosomal nucleic acid gene restriction patterns as potential taxonomic tools. Annales de l'Institut Pasteur/Microbiology 1986, 137B: 165–175.

    CAS  Google Scholar 

  15. Grimont F, Chevrier D, Grimont PAD, Lefevre M, Guesdon JL: Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy. Research in Microbiology 1989, 140: 447–454.

    Article  PubMed  CAS  Google Scholar 

  16. Garaizar J, Kaufmann ME, Pitt TL: Comparison of ribotyping with conventional methods for the type identification ofEnterobacter cloacae. Journal of Clinical Microbiology 1991, 29: 1303–1307.

    PubMed  CAS  Google Scholar 

  17. Grimont F, Grimont PAD: DNA fingerprinting. In: Stackebrandt E, Goodfellow M (ed): Nucleic acid techniques in bacterial systematics. J. Wiley, Chichester, 1991, p. 249–279.

    Google Scholar 

  18. Bingen E, Lambert-Zechovsky N, Leluan G, Desvignes A: Influence de l'ampicilline associée aux aminosides sur l'ecosystème bactérien intestinal de l'enfant. Annales Pharmaceutiques Françaises 1982, 40: 269–280.

    PubMed  CAS  Google Scholar 

  19. Tullus K, Burmann LG: Ecological impact of ampicillin and cefuroxime in neonatal units. Lancet 1989, i: 1405–1407.

    Article  Google Scholar 

  20. Tullus K, Berglund B, Fryklund B, Kuhn I, Burman LG: Epidemiology of fecal strains of the familyEnterobacteriaceae in 22 neonatal wards and influence of antibiotic policy. Journal of Clinical Microbiology 1988, 26: 1166–1170.

    PubMed  CAS  Google Scholar 

  21. Nauciel C, Philippon A, Ronco E, Pilliot J, Guenounou M, Paul G, Brunel D, Outin HD: Septicémies àEnterobacter cloacae etEnterobacter aerogenes. Émergence de variants résistants (céphalosporinase déréprimée) en cours de traitement par des céphalosporines de troisième génération. La Presse Médicale 1985, 14: 673–676.

    PubMed  CAS  Google Scholar 

  22. Gaston MA, Crees Morris JA, Pitt TL: Serotypes and biochemical profiles of British hospital strains ofEnterobacter cloacae in relation to site of infection and antibiotic susceptibility. Journal of Hospital Infection 1987, 10: 17–27.

    Article  PubMed  CAS  Google Scholar 

  23. Irino K, Grimont F, Casin I, Grimont PAD, andthe Brazilian Purpuric Fever Study Group: rRNA gene restriction patterns ofHaemophilus influenzae biogroupAegyptius strains associated with Brazilian purpuric fever. Journal of Clinical Microbiology 1988, 26: 1535–1538.

    PubMed  CAS  Google Scholar 

  24. Bingen E, Denamur E, Lambert-Zechkovsky N, Brahimi N, El Lakany M, Elion J: Rapid genotyping shows the absence of cross-contamination inEnterobacter cloacae nosocomial infections. Journal of Hospital Infection 1992, 21: 95–101.

    Article  PubMed  CAS  Google Scholar 

  25. Verschragen G, Claeys G, Delanghe M, Pattyn P: Serotyping and phage typing to identifyEnterobacter cloacae contaminating total parenteral nutrition. European Journal of Clinical Microbiology and Infectious Diseases 1988, 7: 306–307.

    Article  Google Scholar 

  26. Reybrouck G: Role of the hands in the spread of nosocomial infections. Journal of Hospital Infection 1983, 4: 103–110.

    Article  PubMed  CAS  Google Scholar 

  27. Kjolen H, Andersen BM: Handwashing and disinfection of heavily contaminated hands: effective or in-effective? Journal of Hospital Infection 1992, 21: 61–71.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Microbiologie, Hôpital Jean Verdier, Avenue du 14 Juillet, 93143, Bondy Cedex, France

    I. Poilane, P. Cruaud, J. C. Torlotin & A. Collignon

  2. Service de Pédiatrie, Hôpital Jean Verdier, Avenue du 14 Juillet, 93143, Bondy Cedex, France

    E. Lachassinne & J. Gaudelus

  3. Unité des Enterobactéries, rUnité INSERM 199, Institut Pasteur, 75724, Paris Cedex 15, France

    F. Grimont, P. A. D. Grimont & M. Collin

Authors
  1. I. Poilane
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  2. P. Cruaud
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  3. E. Lachassinne
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  4. F. Grimont
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  5. P. A. D. Grimont
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  6. M. Collin
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  7. J. Gaudelus
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  8. J. C. Torlotin
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  9. A. Collignon
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Poilane, I., Cruaud, P., Lachassinne, E. et al. Enterobacter cloacae cross-colonization in neonates demonstrated by ribotyping. Eur. J. Clin. Microbiol. Infect. Dis. 12, 820–826 (1993). https://doi.org/10.1007/BF02000401

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  • DOI: https://doi.org/10.1007/BF02000401

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