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An enzymatic spectrophotometric assay for inosinic acid

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Summary

An enzymatic, spectrophotometric assay for inosinic acid is described. Inosinic acid is reacted with excess pyrophosphate in the presence of magnesium ions and hypoxanthine guanine phosphoribosyltransferase to form hypoxanthine which is oxidized to uric acid in the presence of xanthine oxidase. Inosinic acid concentration is measured by the increase in absorption at 293 nm at the end of the reaction.

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Authors and Affiliations

  1. Institute of Biological Chemistry and Center of Molecular Biology of C.N.R. Rome, University of Rome, T-00100, Rome, Italy

    C. Salerno & A. Giacomello

  2. Institute of Rheumatology, University of Rome, T-00100, Rome, Italy

    C. Salerno & A. Giacomello

Authors
  1. C. Salerno
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  2. A. Giacomello
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Salerno, C., Giacomello, A. An enzymatic spectrophotometric assay for inosinic acid. Experientia 37, 223–224 (1981). https://doi.org/10.1007/BF01991621

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  • DOI: https://doi.org/10.1007/BF01991621

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