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Use of nonradioactive DNA probes to identify aCampylobacter jejuni strain causing abortion

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Abstract

A case of human abortion due to aCampylobacter infection is reported. Cultures revealed two morphologically different isolates with large and small colonies respectively. Using conventional methods of identification, the large colonies were identified asCampylobacter jejuni and the small colonies asCampylobacter coli. Dot blot hybridization and determination of rDNA restriction fragment patterns revealed that both colony types were the same strain ofCampylobacter jejuni. This observation illustrates the need to use methods other than phenotypic methods when identifying strains ofCampylobacter.

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Authors and Affiliations

  1. Département de Microbiologie Médicale et Moleculaire, Unité de Bactériologie, 37044, Tours Cedex, France

    R. Quentin, C. Martin & A. Goudeau

  2. Maternité du Beffroi, Centre Hospitalier Universitaire Bretonneau, 37044, Tours Cedex, France

    F. Pierre

  3. Laboratoire des Sondes Froides, Institut Pasteur, 25 Rue du Dr. Roux, 75724, Paris Cedex 15, France

    D. Chevrier & J. L. Guesdon

Authors
  1. R. Quentin
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  2. D. Chevrier
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  3. J. L. Guesdon
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  4. C. Martin
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  5. F. Pierre
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  6. A. Goudeau
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Quentin, R., Chevrier, D., Guesdon, J.L. et al. Use of nonradioactive DNA probes to identify aCampylobacter jejuni strain causing abortion. Eur. J. Clin. Microbiol. Infect. Dis. 12, 627–630 (1993). https://doi.org/10.1007/BF01973644

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  • DOI: https://doi.org/10.1007/BF01973644

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