Abstract
WAY-121,520 inhibited human synovial fluid PLA2 (HSF-PLA2) (IC50=4 μM) using arachidonic acid-labeledE. coli as substrate. Further biochemical characterization of WAY-121,520 demonstrated potent inhibition of 5-lipoxygenase (5-LO) activity in the murine macrophage (LTC4, IC50=4nM) and rat PMN (LTB4, IC50=10 nM) and an ability to antagonize LTD4 binding to isolated guinea-pig trachea (pK B=6.0).In vivo anti-inflammatory activity was noted in murine TPA-induced (ED50=91 μg/ear) and arachidonic acid-induced (66% inhibition at 400 μg/ear) ear edema and in leukotriene-dependent antigen-induced bronchoconstriction in the guinea pig (73% inhibition at 50 mg/kg, p.o.). WAY-121,520 represents a novel series of indomethacin-based inhibitors of PLA2 with anti-inflammatory activity resulting from a combination of biochemical activities (inhibition of 5-LO and PLA2 and LTD4 antagonism). This agent may provide added therapeutic efficacy over more selective inhibitors.
Similar content being viewed by others
References
L. Kaplan, J. Weiss and P. Elsbach,Low concentrations of indomethacin inhibit phospholipase A 2 of rabbit polymorphonuclear leukocytes. Proc. Natl. Acad. Sci. USA75, 2955–2958 (1978).
L. A. Marshall, J. Bauer and J. Y. Chang,Evaluation of antirheumatic drugs for their effect in vitro on purified human synovial fluid phospholipase A 2. J. Rheumatol.18, 59–65 (1991).
A. F. Kreft, A. A. Failli, J. H. Musser, D. M. Kubrak, A. L. Banker, R. Steffan, C. A. Demerson, J. A. Nelson, U. S. Shah, W. Gray, L. A. Marshall, D. Holloway, R. Sturn, R. P. Carlson, J. Berkenkopf, D. Grimes, B. M. Weichman and J. Y. Chang,Conversion of a cyclooxygenase (CO) inhibitor into a 5-lipoxygenase (LO) inhibitor: A general route to novel orally active anti-inflammatory and anti-allergy drugs. Drugs Exp. Clin. Res.17, 381–387 (1991).
A. Kreft, J. Nelson, J. Musser, A. Failli, U. Shah, D. Kubrak, A. Banker, R. Steffan, G. Schiehser, R. Sturm, D. Holloway, J. Bauer, M.-L. Sung, L. Marshall and K. Glaser,Structure-activity relationships leading to WAY-121,520, a tris aryl-type, indomethacin-based, phospholipase A 2 /5-lipoxygenase (5-LO) inhibitor. Agents and Actions (in press).
K. B. Glaser, Y. W. Lock and J. Y. Chang,PAF and LTB 4 biosynthesis in the human neutrophil: Effects of putative inhibitors of phospholipase A 2 and specific inhibitors of 5-lipoxygenease. Agents and Actions34, 89–92 (1991).
O. Arunlakshana and H. O. Schild,Some quantitative uses of drug antagonists. Brit. J. Pharmacol.14, 48–58 (1959).
A. M. S. Mayer, K. B. Glaser and R. S. Jacobs,Regulation of eicosanoid biosynthesis in vitro and in vivo by the marine natural product manoalide: A potent inactivator of venom phospholipase A 2 J. Pharm. Exp. Ther.244, 871–878 (1988).
K. B. Glaser, M. S. deCarvalho, R. S. Jacobs, M. R. Kernan and D. J. Faulkner,Manoalide: Structure-activity studies and definition of the pharmacophore for phospholipase A 2 inactivation. J. Pharm. Exp. Ther.36, 782–788 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Glaser, K.B., Carlson, R.P., Sung, A. et al. Pharmacological characterization of WAY-121,520: A potent anti-inflammatory indomethacin-based inhibitor of 5-lipoxygenase (5-LO)/phospholipase A2 (PLA2). Agents and Actions 39 (Suppl 1), C30–C32 (1993). https://doi.org/10.1007/BF01972711
Issue Date:
DOI: https://doi.org/10.1007/BF01972711