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Effects of bradykinin and leukotrienes B4 and D4 on the bovine brochial arteryin vitro: Role of the endothelium

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Abstract

The effects of bradykinin, leukotriene (LT) B4 and LTD4 on the bovine bronchial artery were studied using isolated, perfused vessel segments. Intrapulmonary bronchial arteries were precontracted to the EC50 of 5-hydroxytryptamine in the organ bath, after which increasing concentrations of bradykinin, LTB4 or LTD4 were perfused through the vessel lumen. In arteries with intact endothelium, bradykinin induced a concentration-dependent relaxation beginning at 1 pmol/l (p<0.01), whereas LTD4 and LTB4 had no significant effect. Indomethacin (10 μmol/l) significantly (p<0.01) inhibited bradykinin-induced endothelium-denuded arteries bradykinin had no observed effect, whereas LTB4 and LTD4 significantly increased tone with threshold concentrations of 50 pmol/l (p<0.025) and 5 pmol/l (p<0.05), respectively. The data indicate that in the bronchial artery: 1) bradykinin is a potent vasodilator; 2) bradykinin-induced relaxation is endothelium-dependent and only partially blocked by a cyclooxygenase inhibitor; 3) removal of the endothelium unmasks a potent contractile effect of LTB4 and LTD4.

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Authors and Affiliations

  1. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    W. R. Tracey & P. Eyre

  2. Department of Pharmacology and Toxicology, University of Western Ontario, N6A 5C1, London, Ontario, Canada

    W. R. Tracey

  3. Department of Veterinary Biosciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, 24061, Blacksburg, Virginia, USA

    P. Eyre

Authors
  1. W. R. Tracey
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  2. P. Eyre
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Tracey, W.R., Eyre, P. Effects of bradykinin and leukotrienes B4 and D4 on the bovine brochial arteryin vitro: Role of the endothelium. Agents and Actions 25, 195–204 (1988). https://doi.org/10.1007/BF01969112

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  • DOI: https://doi.org/10.1007/BF01969112

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