Summary
(S)-9-(2,3-dihydroxypropyl) adenine (DHPA), D-eritadenine and some other open-chain nucleoside analogues, which exhibit adverse biological effects in microorganisms, plants and animals, cause pronounced inhibition of intestinal phosphatases in the hemipteran insectPyrrhocoris apterus. The rate of p-nitrophenylphosphate hydrolysis by homogenates from intestinal epithelium and Malpighian tubules was inhibited up to 94% by 2–10 millimolar concentrations of these drugs. This effect is stronger than that of sodium fluoride, which is recognized as a common inhibitor of phosphatase. We conclude that inhibition of phosphatase activity in the digestive and excretory organs may be responsible for the previously reported massive excretion of phosphorylated derivatives of the nucleoside analogues after their oral administration to insects.
References
DeClercq, E., Bergstrom, D. E., Holý, A., and Montgomery, J. A., Antiviral Res.4 (1984) 119.
Beneš, K., Holý, A., Melichar, O., and Rosenberg, I., Biol. Plant.28 (1986) 47.
Sláma, K., Holý, A., and Votruba, I., Ent. exp. appl.33 (1983) 9.
Jelínek, R., Holý, A., and Votruba, I., Teratology24 (1981) 267.
Holý A., Votruba, I., Merta, A., DeClercq, E., Jelínek, R., Sláma, K., Beneš, K., and Melichar, O., Biological consequences of S-adenosyl-L-homocysteinase inhibition, p. 397. Humana Press Inc., Clifton (USA) 1986.
Votruba, I., and Holý, A., Coll. Czech. chem. Commun.47 (1982) 167.
Votruba, I., Holý, A., Rosenberg, I., and Sláma, K., Insect Biochem.15 (1985) 631.
Holý, A., Rosenberg, I., Votruba, I., and Sláma, K., Biol. Chem. Hoppe Seyler366 (1985) 355.
Stay, B., J. Morph.105 (1959) 457.
Linhardt, K., and Walter, K., Methods of Enzymatic Analysis, p. 146. Academic Press, New York 1965.
Votruba, I., and Holý, A., Coll. Czech. chem. Commun.45 (1980) 3039.
Sláma, K., and Holý, A., Acta ent. bohemoslov.85 (1988) 94.
Gelbič, I., Tonner, M., and Holý, A., Acta ent. bohemoslov.81 (1984) 46.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Němec, V., Sláma, K. Inhibition of phosphatase by open-chain nucleoside analogues in insects. Experientia 45, 148–150 (1989). https://doi.org/10.1007/BF01954853
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01954853