Abstract
A cytochrome P450, termed P450lpr, is the major P450 responsible for pyrethroid resistance in the Learn-PyR (LPR) strain of house fly. Recently, the putative gene (CYP6D1) coding for P450lpr has been sequenced from the LPR and aabys strains of house fly. Allele-specific polymerase chain reaction (ASPCR) was used for linkage group analysis with backcross progeny from the wild type LPR strain and a multiple marker strain (aabys). We found thatCYP6D1 is linked to chromosome 1. The possible role of regulatory or modifying genes responsible for elevated P450lpr expression is discussed in relation to the chromosomal linkage ofCYP6D1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nebert, D. W., A. Rev. Biochem.56 (1987) 945.
Hodgson, E., in: Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol. 11, p. 225. Eds G. A. Kerkut and L. I. Gilbert. Pergamon Press, New York 1985.
Oppenoorth, F. J., in: Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol. 12, p. 731. Eds G. A. Kerkut and L. I. Gilbert. Pergamon Press, New York 1985.
Scott, J. G., in: Handbook of Pest Management, vol. 2, p. 663. Ed. D. Pimentel CRC press, Boca Raton 1991.
Scott, J. G., Shono, T., and Georghiou, G. P., Experientia40 (1984) 1416.
Tsukamoto, M., in: Pest Resistance to Pesticides, p. 71. Eds G. P. Georghiou and T. Saito. Plenum Press, New York 1983.
Plapp, F. W. Jr., Pestic. Biochem. Physiol.22 (1984) 194.
Wheelock, G. D., and Scott, J. G., Pestic. Biochem. Physiol.43 (1992) 67.
Scott, J. G., and Georghiou, G. P., J. econ. Ent.78 (1985) 316.
Wheelock, G. D., and Scott, J. G., Insect. Biochem.19 (1989) 481.
Georghiou, G. P., Expl Parasit.26 (1969) 224.
Takada, T., and Konami, R., in: Handbook of Gene Technology-suppl, Experimental Medicine, p. 41. Eds M. Muramatsu and H. Okayama. Yodosha, Tokyo 1992.
Wu, D. Y., Ugozzoli, L., Pal, B. K., and Wallace, R. B., Proc. natl Acad. Sci. USA86 (1989) 2757.
Cha, R. S., Zarbl, H., Keohavong, P., and Thilly, W. G., PCR Meth. Applic.2 (1992) 14.
Mullin, C. A., and Scott, J. G., in: Molecular Mechanisms of insecticide Resistance: Diversity among Insects, p. 1. Eds C. J. Mullin and J. G. Scott. American Chemical Society, Washington DC. 1992.
Scott, J. G., and Georghiou, G. P., Biochem. Genet.24 (1986) 25.
Wheelock, G. D., and Scott, J. G., Pestic. Biochem. Physiol.38 (1990) 130.
Carino, F. A., Koener, J. F., Plapp, F. W. Jr., and Feyereisen, R., Insect Biochem. molec. Biol.4 (1994) 411.
Carino, F. A., Koener, J. F., Plapp, F. W. Jr., and Feyereisen, R., in: Molecular Mechanisms of Insecticide Resistance: Diversity among Insects, p. 31. Eds C. J. Mullin and J. G. Scott. American Chemical Society, Washington DC 1992.
Tomita, T., and Scott, J. G., Insect Biochem. molec. Biol. (1994) in press.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, N., Tomita, T. & Scott, J.G. Allele-specific PCR reveals thatCYP6D1 is on chromosome 1 in the house fly,Musca domestica. Experientia 51, 164–167 (1995). https://doi.org/10.1007/BF01929363
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01929363