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Gonadotropins I and II do not stimulate thein vitro secretion of 17α,20β-dihydroxy-4-pregnen-3-one 20-sulphate by rainbow trout gonads during final sexual maturation

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Abstract

Thein vitro secretion of 17α,20β-dihydroxy-4-pregnen-3-one 20-sulphate (17α,20β-P-sulphate) and the free steroid 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-P), by rainbow trout (Oncorhynchus mykiss) gonads, in response to gonadotropin (GTH) I and GTH II, were studied during the final stages of sexual maturation. Substantial amounts of 17α,20β-P-sulphate were produced, by both mature ovaries and testes, indicating considerable 20β-hydroxysteroid sulphotransferase (20β-HST) activity within these tissues. In the post-ovulatory ovary the level of 17α,20β-P-sulphate (36.6 ng ml−1) greatly exceeded that of 17α,20β-P (8.59 ng ml−1). The amount of 17α,20β-P-sulphate produced in incubations of both mature ovary and testes was unaffected by either GTH I or GTH II treatment at physiological concentrations up to 100 ng ml−1. Similarly, incubations of maturing ovary and testes, treated with GTH I or GTH II, in the presence of added 17α,20β-P at 100 ng ml−1 of medium, produced levels of 17α,20β-P-sulphate that were similar to those of the controls. In incubations of mature ovarian follicles at the stages of germinal vesicle breakdown and preovulation, both GTHs significantly stimulated secretion of 17α,20β-P, although GTH II was always more potent than GTH I. GTH II significantly elevated the levels of 17α,20β-P in testicular incubations from mature males more than 4-fold relative to GTH I and controls, which did not differ from one another.

In conclusion, 20β-HST, the enzyme responsible for the sulphate conjugation of 17α,20β-P, was found to be active in the ovaries and testes of rainbow troutin vitro. However, the levels of this enzyme do not appear to be regulated by either GTH I or GTH II.

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Author notes

  1. J. J. Nagler

    Present address: Maurice Lamontagne Institute, Fisheries and Oceans, G5H 3Z4, Mont-Joli, Quebec, Canada

Authors and Affiliations

  1. Department of Biology and Biochemistry, Brunel University, UB8 3PH, Uxbridge, Middlesex, UK

    J. J. Nagler, C. R. Tyler & J. P. Sumpter

  2. Ministry of Agriculture, Fisheries and Food, Directorate of Fisheries Research, Fisheries Laboratory, NR33 OHT, Lowestoft, Suffolk, UK

    A. P. Scott

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  1. J. J. Nagler
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  4. J. P. Sumpter
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Nagler, J.J., Scott, A.P., Tyler, C.R. et al. Gonadotropins I and II do not stimulate thein vitro secretion of 17α,20β-dihydroxy-4-pregnen-3-one 20-sulphate by rainbow trout gonads during final sexual maturation. Fish Physiol Biochem 15, 149–156 (1996). https://doi.org/10.1007/BF01875594

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  • DOI: https://doi.org/10.1007/BF01875594

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