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Gradient-enhanced 3D NOESY-HMQC spectroscopy

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Summary

A gradient-enhanced 3D NOESY-HMQC experiment is presented and applied to15N-labeled Mnt repressor (1–76) in1H2O. Both coherence selection and1H2O suppression are achieved using gradients. A singlescan experiment can be recorded for each time increment, which greatly reduces recording time with respect to conventional methods using phase cycling. This can be of use for kinetic studies and for relatively unstable molecules.

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Author notes

  1. Geerten W. Vuister

    Present address: Laboratory of Chemical Physics, Building 2, NIDDK, National Institutes of Health, 20892, Bethesda, MD, USA

Authors and Affiliations

  1. Bijvoet Center for Biomolecular Research, Department of Chemistry, University of Utrecht, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    Geerten W. Vuister, Rolf Boelens, Robert Kaptein & Maurits Burgering

  2. Department of Pharmacology, Georgetown University Medical School, 4 Research Court, 20850, Rockville, MD, USA

    Peter C. M. van Zijl

Authors
  1. Geerten W. Vuister
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  2. Rolf Boelens
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  3. Robert Kaptein
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  4. Maurits Burgering
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  5. Peter C. M. van Zijl
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Vuister, G.W., Boelens, R., Kaptein, R. et al. Gradient-enhanced 3D NOESY-HMQC spectroscopy. J Biomol NMR 2, 301–305 (1992). https://doi.org/10.1007/BF01875323

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  • DOI: https://doi.org/10.1007/BF01875323

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