Summary
The strength of the long-range electrostatic repulsion forces on HeLa cells is measured by agglutinative titration using low molecular weight polylysine (M.W. 11,000). Repulsion forces, found to be present on the smaller HeLa cells from density-inhibited suspension cultures, are weakened by incubation of the cells in hypotonic NaCl solutions. Repulsion forces, found to be absent on the larger cells from fast growing cultures, can be induced on these cells by incubation in hypertonic NaCl solutions. Both effects of anisotonicity are reversible, and disappear on restoration of the medium to normal tonicity. Induction of repulsion forces on fast growing cells is prevented by previous treatment of the cells with neuraminidase. Neuraminidase also abolishes repulsion on density-inhibited cells. It is proposed that alterations of the cells size, produced by anisotonicity or occurring during growth in isotonic suspension medium, affect mutual cell adhesiveness by modifying the strength of the repulsion forces generated by cell surface sialic acids.
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References
Brooks, D.E., Seaman, G.V.F. 1972. Electroviscous effect in dextrane-erythrocyte suspensions.Nature, New Biol. 238:251
Buck, C.A., Glick, M.C., Warren, L. 1971. Effect of growth on the glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells.Biochemistry 10:2176
Curtis, A.S.G. 1973. Cell adhesion.Prog. Biophys. Mol. Biol. 27:316
Deman, J.J., Bruyneel, E.A. 1974. Evidence for long-range electrostatic repulsion between HeLa cells.Exp. Cell Res. 89:206
Deman, J.J., Bruyneel, E.A. 1975. Intercellular adhesiveness and neuraminidase effect following release from density inhibition of cell growth.Biochem. Biophys. Res. Commun. 62:895
Deman, J.J., Vakaet, L.C., Bruyneel, E.A. 1976. Cell size and mutual cell adhesion. I. Increase in mutual adhesiveness of HeLa cells from density-inhibited suspension cultures by hypotonic treatment.J. Membrane Biol. 26:189
Glick, M.C., Buck, C.A. 1972. Glycoproteins from the surface of metaphase cells.Biochemistry 12:85
Jan, K.M., Chien, S. 1973. Role of surface electric charge in red blood cell interactions.J. Gen. Physiol. 61:638
Jones, G.E. 1974. Intercellular adhesion: Modification by dielectric properties of the medium.J. Membrane Biol. 16:297
Marikovsky, Y., Inbar, M., Danon, D., Sachs, L. 1974. Distribution of surface charge and concanavalin-A binding sites on normal and transformed cells.Exp. Cell Res. 89:359
Muramatsu, T., Atkinson, P.H., Nathenson, S.G., Ceccarini, C. 1973. Cell-surface glycopeptides: Growth-dependent changes in the carbohydrate-peptide linkage region.J. Mol. Biol. 80:781
Nicolson, G.L. 1973. Anionic sites on human erythrocyte membranes. I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles.J. Cell Biol. 57:373
Nicolson, G.L., Painter, R.G. 1973. Anionic sites on human erythrocyte membranes. II. Antispectrin-induced transmembrane aggregation of the binding sites for positively charged colloidal particles.J. Cell Biol. 59:395
Pethica, B.A. 1961. The physical chemistry of cell adhesion.Exp. Cell Res. 8:123
Weiss, L. 1974. The topography of cell surface sialic acids and their possible relationship to specific cell interactions.Behring Inst. Mitt. 55:185
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Deman, J.J., Vakaet, L.C. & Bruyneel, E.A. Cell size and mutual cell adhesion. J. Membrain Biol. 26, 205–215 (1976). https://doi.org/10.1007/BF01868874
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DOI: https://doi.org/10.1007/BF01868874