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Hormone dependence of a mouse mammary tumor line inducedin vivo by medroxyprogesterone acetate

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Summary

The administration of MPA to virgin female BALB/c mice led to the development of mammary adenocarcinomas, which in furtherin vivo transplants gave rise to both MPA-dependent and MPA-independent lines. In this paper we chose one of the MPA-dependent lines with high contents of estrogen (ER) and progesterone (PR) receptors, and were able to demonstrate that a) the growth of these tumors could be manipulated by the administration or the withdrawal of the hormonal supply;b) PR were down-regulated in MPA-treated mice; c) progesterone had the same stimulatory effect as MPA on tumor growth; d) tumors did not grow in estrogen-treated mice; e) tumor growth was much lower in males than in females; f) the presence of the ovaries had a positive influence on tumor growth, even in the presence of MPA; g) the withdrawal of progestin pellets in ovariectomized mice usually led to complete remissions followed by regrowth of the tumors after several weeks; and h) the regrowing tumors maintained their steroid receptor pattern and (in 3 out of 4 cases) their hormone-dependent behavior in further passages.

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Abbreviations

DMBA:

7,12 dimethylbenz(a)anthracene

EB:

estradiol benzoate

ER:

estrogen receptors

MPA:

medroxyprogesterone acetate

P:

progesterone

PR:

progesterone receptors

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Authors and Affiliations

  1. Instituto de Investigaciones Hematológicas and Instituto de Estudios Oncológicos, Academia Nacional de Medicina and Instituto de Biologiay Medicina Experimental, Las Heras 3092, 1425, Buenos Aires, Argentina

    Edith Kordon, Claudia Lanari, Roberto Meiss, Patricia Elizalde, Eduardo Charreau & Christiane Dosne Pasqualini

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  1. Edith Kordon
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  2. Claudia Lanari
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  3. Roberto Meiss
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  4. Patricia Elizalde
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  6. Christiane Dosne Pasqualini
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Kordon, E., Lanari, C., Meiss, R. et al. Hormone dependence of a mouse mammary tumor line inducedin vivo by medroxyprogesterone acetate. Breast Cancer Res Tr 17, 33–43 (1990). https://doi.org/10.1007/BF01812682

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