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Immunoscintigraphic localization of renal tumours in an extracorporeal perfusion model with a monoclonal antibody against γ-glutamyltransferase

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Summary

Monoclonal antibody 138H11 against human γ-glutamyltransferase has been shown to react immunohistochemically with 98% of all tested clear-cell type and chromophilic renal cell carcinomas, but not with renal chromophobic carcinomas, Duct-Bellini carcinomas or oncocytomas. In normal kidney the target epitopes of mAb 138H11 are located in the luminal brush-border membrane of proximal tubule cells, whereas in renal carcinomas the epitopes are found surrounding the whole tumour cells. These results form the basis of the present immunoscintigraphic study designed to evaluate mAb 138H11 in an extracorporeal perfusion model. Immediately after nephrectomy, human tumour-bearing kidneys were perfused with99mTc-labelled mAb 138H11 in Euro-Collins solution. High specific uptake in 4/4 renal clear cell carcinomas could be demonstrated by planar immunoscintigraphy and single-photon-emission computed tomography, “regions of interest” investigation and immunohistochemistry. In contrast, a perfused oncocytoma showed up as an unlabelled lesion. The results indicate a possible use for mAb 138H11 in immunoscintigraphy or even therapy, provided high tumour uptake can be confirmed in patients.

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Author information

Author notes

  1. Peter Fischer

    Present address: Department of Immunology, The Scripps Research Institute, 10 666 North Torrey Pines Road, 92 037, La Jolla, CA, USA

  2. Wieland Boeckmann

    Present address: Urologische Klinik RWTH, Pauwelstr. 30, W-5100, Aachen, FRG

Authors and Affiliations

  1. Department of Nephrology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany

    Peter Fischer, Monika Tauber & Jürgen E. Scherberich

  2. Department of Nuclear Medicine, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany

    Richard P. Baum

  3. Department of Urology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany

    Wieland Boeckmann

  4. Department of Pathology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany

    Stefan Weier

Authors
  1. Peter Fischer
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  6. Jürgen E. Scherberich
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Fischer, P., Baum, R.P., Tauber, M. et al. Immunoscintigraphic localization of renal tumours in an extracorporeal perfusion model with a monoclonal antibody against γ-glutamyltransferase. Cancer Immunol Immunother 35, 283–288 (1992). https://doi.org/10.1007/BF01789336

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  • DOI: https://doi.org/10.1007/BF01789336

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