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Metastatic instability of murine tumor metastases: Dependence on tumor type

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Abstract

Fifteen metastatic lines derivedin vivo from three syngeneic murine tumors, the sarcomas SA-NH and SA-4020 and the hepatic carcinoma HCA-I, were assessed for their stability of metastasis formation upon isotransplantation for several successive generations in syngeneic animals. Change in the metastatic phenotype was actively encouraged by a new procedure, the artificial selection for increased or decreased metastasis formation. Metastatic instability was dependent on tumor type, with five of six lines of tumor SA-NH, one of four lines of tumor SA-4020, and possibly one of five lines of HCA-I changing in lung metastasis formation. The instability of lung metastasis formation was also assessed by analyzing changes in the variance of the lines. Concomitant with a change in metastatic potential for lung metastasis, we observed a similar change for abdominal lymph node metastasis. We also report the selection of a less metastatic line. The variance of lung metastasis increased significantly only in the SA-NH lines. The instability of metastasis formation was attributed to genetic instability of metastatic cell lines.

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Authors and Affiliations

  1. Department of Experimental Radiotherapy, The University of Texas M. D. Anderson Hospital and Tumor Institute at Houston, 77030, Houston, Texas, USA

    John P. Volpe & Luka Milas

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  1. John P. Volpe
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  2. Luka Milas
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Volpe, J.P., Milas, L. Metastatic instability of murine tumor metastases: Dependence on tumor type. Clin Exp Metast 6, 333–346 (1988). https://doi.org/10.1007/BF01753580

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  • DOI: https://doi.org/10.1007/BF01753580

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