Abstract
The aim of this study was to develop a simple, reliable, and inexpensive in-house system for routine species identification of staphylococci in clinical practice. The system combines 15 key tests (including carbohydrate fermentation) performed in micro-well strips and antimicrobial disk diffusion susceptibility tests performed on standardised paper disk method antibiotic sensitivity medium agar. Twenty-eight Staphylococcal reference strains belonging to 18 different species were correctly identified using this in-house system. A total of 291 clinical staphylococci isolates were evaluated with the in-house system and a conventional identification scheme. The in-house system identified 281 (96.6%) of these 291 isolates. Eleven different species were recognised. The five species most frequently identified wereStaphylococcus epidermidis (48.6%),Staphylococcus aureus (27.8%),Staphylococcus haemolyticus (8.2%),Staphylococcus hominis (5.7%), andStaphylococcus warneri (5.3%). There was an agreement of 86.3% between the species identification obtained with the in-house system and the conventional identification scheme. All coagulase-negative isolates initially identified as species other thanStaphylococcus epidermidis as well as indistinctly identified isolates were also evaluated with a commercial identification system. The agreement between species identification obtained with the inhouse system and the commercial system for 101 identified isolates was 73%. Several isolates that were difficult to distinguish with the conventional scheme and/or the commercial system were identified with the aid of the antimicrobial susceptibility test included in the in-house system. The described test scheme should be of value for identification of clinically significant staphylococci species.
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Monsen, T., Rönnmark, M., Olofsson, C. et al. An inexpensive and reliable method for routine identification of staphylococcal species. Eur. J. Clin. Microbiol. Infect. Dis. 17, 327–335 (1998). https://doi.org/10.1007/BF01709455
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DOI: https://doi.org/10.1007/BF01709455