Abstract
The 1 μg oxacillin disk diffusion screening test was performed on 1516 recent clinical isolates ofStreptococcus pneumoniae obtained in a 1994–1995 U.S. surveillance study and the results compared to penicillin MICs determined using a standardized broth microdilution method. The oxacillin disk screening method failed to distinguish penicillin-resistant strains from those that were intermediately susceptible. Furthermore, a high percentage (11.1 %) of penicillin-susceptible strains, for which MICs of penicillin were usually 0.06 or 0.03 μg/ml, yielded zone diameters of ≤ 19 mm with the oxacillin screen test and thus would have been falsely categorized as being resistant to penicillin.
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References
Dixon JMS, Lipinski AE, Graham MEP: Detection and prevalence of pneumococci with increased resistance of penicillin. Canadian Medical Association Journal 1977, 117: 1159–1161.
Jacobs MR, Koornhof HJ, Robins-Browne RM, Stevenson CM, Vermaak ZA, Freiman I, Miller GB, Witcomb MA, Isaacson M, Ward JI, Austrian R: Emergence of multiply resistant pneumococci. New England Journal of Medicine 1978, 299: 735–740.
National Committee for Clinical Laboratory Standards: Methods performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A6. NCCLS, Villanova, PA, 1996.
College of American Pathologists: Bacteriology survey, D-C. College of American Pathologists, Waukegan, IL, 1995.
Appelbaum PC: Antimicrobial resistance inStreptococcus pneumoniae. Clinical Infectious Diseases 1992, 15: 77–83.
Doern GV, Brueggemann A, Holley HP Jr, Rauch AM: Antimicrobial resistance ofStreptococcus pneumoniae recovered from outpatients in the United States during the winter months of 1994 to 1995: results of a 30-center national surveillance study. Antimicrobial Agents and Chemotherapy 1996, 40: 1208–1213.
Klugman KP: Pneumococcal resistance to antibiotics. Clinical Microbiology Reviews 1990, 3: 171–196.
National Committee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A4. NCCLS, Villanova, PA, 1996.
Jacobs MR, Caspar MN, Robins-Browne RM, Koornhof HJ: Antimicrobial susceptibility testing of pneumococci. Journal of Antimicrobial Chemotherapy 1980, 6: 53–64.
Swenson JM, Hill BC, Thornsberry C: Screening pneumococci for penicillin resistance. Journal of Clinical Microbiology 1986, 24: 749–752.
Johnson AP, Warner M, George RC, Boswell TC, Fraise AP, Manek N: Oxacillin resistant pneumococci sensitive to penicillin. Lancet 1993, 341: 1222.
Dowson CG, Johnson AP, Cercenado E, George RC: Genetics of oxacillin resistance in clinical isolates ofStreptococcus pneumoniae that are oxacillin resistant and penicillin susceptible. Antimicrobial Agents and Chemotherapy 1994, 38: 49–53.
National Committee for Clinical Laboratory Standards: Development of in vitro susceptibility testing criteria and quality control parameters. Approved standard M23-A. NCCLS, Villanova, PA, 1994.
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Doern, G.V., Brueggemann, A.B. & Pierce, G. Assessment of the oxacillin disk screening test for determining Penicillin resistance inStreptococcus pneumoniae . Eur. J. Clin. Microbiol. Infect. Dis. 16, 311–314 (1997). https://doi.org/10.1007/BF01695637
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DOI: https://doi.org/10.1007/BF01695637