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Assessment of inhibition kinetics of the growth of strain P5 on pentachlorophenol under steady-state conditions in a nutristat

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Abstract

A bacterium degrading pentachlorophenol (PCP) as the only source of carbon and energy was grown in a “nutristat”, i.e., a continuous culture with on-line measurement and control of the substrate concentration. We improved the PCP nutristat by incorporation of a personal computer with a proportional integral derivative (PID) algorithm for controlling the medium feed pump. The controlled value deviated from the average (set-point) value by 1% maximally. In the PCP nutristat (30°C), the steadystate dilution rate, and hence, specific growth rate, showed a maximum value of 0.142±0.004 h-1 at set-point PCP concentrations between 37 and 168 μM. At PCP concentrations above 168 μM, the steady-state growth rate decreased because of inhibition. The growth yield coefficient was not seriously affected by the PCP concentration, suggesting that uncoupling was not the inhibitory mechanism. It was concluded that the PCP nutristat is very useful for establishing steady-state conditions that maintain growth-inhibitory PCP concentrations and high cell concentrations, conditions for which the chemostat is not suitable.

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Abbreviations

MCA :

Metabolic control analysis

NTA :

Nitrilotriacetic acid

PCP :

Pentachlorophenol

PID :

Proportional integral derivative

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Authors and Affiliations

  1. Laboratory for Waste Materials and Emissions, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands

    Michiel Rutgers, Daniel D. Gooch, Anton M. Breure & Johan G. Van Andel

Authors
  1. Michiel Rutgers
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  2. Daniel D. Gooch
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  3. Anton M. Breure
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  4. Johan G. Van Andel
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Correspondence to Michiel Rutgers.

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Rutgers, M., Gooch, D.D., Breure, A.M. et al. Assessment of inhibition kinetics of the growth of strain P5 on pentachlorophenol under steady-state conditions in a nutristat. Arch. Microbiol. 165, 194–200 (1996). https://doi.org/10.1007/BF01692861

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  • DOI: https://doi.org/10.1007/BF01692861

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