Summary
Borrelia burgdorferi was cultivated from three cerebrospinal fluid (CSF) samples of children (aged three and a half, four and a half and eight years) who were admitted to the hospital because of acute facial palsy, aseptic meningitis, and aseptic meningitis plus facial palsy. CSF was taken on day one in two cases and on day two in the remaining case after onset of symptoms. All three strains showed a very similar SDS-PAGE pattern, without an OspB and 20kD band. However, of nine monoclonal antibodies (Moab) raised againstB. burgdorferi B31, the Moab H5332 recognized two strains, one of them very weakly, and the flagella specific Moabs H9724, H605, and H6TS (less intensively) recognized all strains. This preliminary characterization reveals heterogeneity among CSFBorrelia isolates of cases from a very close geographic area.
Zusammenfassung
Borrelia burgdorferi wurde aus drei Liquorproben von Kindern (Alter: dreieinhalb, viereinhalb und acht Jahre) angezüchtet, welche aufgrund akuter Fazialisparese, aseptischer Meningitis und aseptischer Meningitis mit Fazialisparese ins Krankenhaus eingeliefert worden waren. Liquor wurde in zwei Fällen am ersten Tag, in einem am zweiten Tag nach Auftreten der Symptome gewonnen. Die drei Borrelien-Isolate waren in ihrem SDS-PAGE-Muster sehr ähnlich. Sie unterschieden sich jedoch in ihrer Reaktion mit neun monoklonalen Antikörpern (MoAb), die gegen den TypstammB. burgdorferi B31 entwickelt worden waren. MoAb H5332 erkannte zwei Stämme, einen davon in einer sehr schwachen Reaktion; die Flagella-spezifischen MoAbs H9724, H605 und H6TS, dieser weniger intensiv, erkannten alle Stämme. Diese vorläufige Charakterisierung zeigt Heterogenität unterB. burgdorferi-Liquor-Isolaten von Patienten aus der gleichen geografischen Region.
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Khanakah, G., Stanek, G., Millner, M.M. et al. Preliminary characterization of Borrelia burgdorferi CSF isolates. Infection 19, 287–288 (1991). https://doi.org/10.1007/BF01644969
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DOI: https://doi.org/10.1007/BF01644969