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Staphylococcus aureus in patients with cystic fibrosis: An epidemiological analysis using a combination of traditional and molecular methods

Epidemiologische Analyse des Auftretens von Staphylococcus aureus bei Mukoviszidosepatienten mit Hilfe konventioneller und molekularer Methoden

  • Addendum III
  • Staphylococcal Aspects Of Cystic Fibrosis
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Summary

An epidemiological analysis ofStaphylococcus aureus was conducted in a study group of 157 cystic fibrosis patients cultured over a 30-month period. The resultingS. aureus isolates were categorized by bacteriophage type, plasmid profile, and (in some instances) chromosomal restriction fragment pattern of the culture-positive patients withS. aureus (34 of 157) 44% only were sporadically infected while 68% shared identical strains with one or more other patients. Six patients exhibited persistent infection (for up to ten months) which, in three individuals, occurred as cycles of carriage and reappearance. By contributing toward our understanding of the persistence and spread ofS. aureus in cystic fibrosis patients these data should aid in clarifying the role this organism may play in the course of the disease.

Zusammenfassung

Das Auftreten vonStaphylococcus aureus wurde bei 157 Mukoviszidosepatienten über 30 Monate hin analysiert. Dazu wurden dieS. aureus-Isolate nach Lysotyp, Plasmidmuster sowie in einigen Fällen dem Restriktionsmuster ihrer chromosomalen DNS klassifiziert. Danach konnte in 44% aller Patienten, in deren SputaS. aureus nachweisbar war, bei aufeinanderfolgenden Episoden ein Wechsel desS. aureus-Typs beobachtet werden. IdentischeS. aureus-Typen wurden bei 68% der Patienten gefunden, wobei derselbe Typ in bis zu fünf verschiedenen Patienten auftrat. Bei sechs Patienten persistierte derselbeS. aureus-Stamm (bis zu zehn Monate), bei drei unter ihnen war er zwischendurch nicht mehr nachweisbar. Die Ergebnisse weisen auf die Möglichkeit der Ausbreitung vonS. aureus-Stämmen unter Mukoviszidosepatienten hin.

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Authors and Affiliations

  1. Department of Medical Microbiology, Creighton University School of Medicine, 719 N. 24th Street, 68178, Omaha, Nebraska, USA

    R. V. Goering

  2. Max von Pettenkofer-Institut für Hygiene und Medizinische Microbiologie der Ludwig-Maximilians-Universität München, Pettenkoferstraße 9 a, D-8000, München 2

    A. Bauernfeind &  Barbara Przyklenk

  3. Institut für Mikrobiologie und Immunologie der Universität, Bonn, FR Germany

    W. Lenz

Authors
  1. R. V. Goering
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  2. A. Bauernfeind
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  3. Barbara Przyklenk
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  4. W. Lenz
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Goering, R.V., Bauernfeind, A., Przyklenk, B. et al. Staphylococcus aureus in patients with cystic fibrosis: An epidemiological analysis using a combination of traditional and molecular methods. Infection 18, 57–60 (1990). https://doi.org/10.1007/BF01644187

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