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A new histological procedure for re-evaluation of the serological test forHelicobacter pylori

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Abstract

To re-evaluate the accuracy of the serological test forHelicobacter pylori, fixation of biopsy specimens with Carnoy's solution (preserving the mucous layer in tissue preparations) followed by immunohistochemical staining (a new histological procedure) was used as the reference histological method instead of 10% formalin fixation followed by hematoxylin-eosin staining (the conventional histological procedure). Biopsy specimens (antrum and body) from 114 patients with gastritis (including non-ulcer dyspepsia) or gastric and/or duodenal ulcers were obtained by endoscopy and used for both bacteriological culture and histological examination. Serum samples were taken from all patients at the time of endoscopy. The serum levels of specific IgG and IgA antibodies forHelicobacter pylori were measured by commercial enzyme immunoassay kits. The reliability of the IgG and IgA measurements was evaluated by analyzing receiver operating characteristic curves obtained using the two histological procedures. With the conventional histological procedure as the reference, the sensitivity and specificity levels of the serological test were 87.2% and 82.1%, respectively. With the new histological procedure as reference, sensitivity and specificity were 94% and 96.7%, respectively. The insufficient accuracy reported for the serological test could be due to false-positive or false-negative results obtained when the conventional histological procedure is used as the reference. The new histological procedure used here revealed that the serological test forHelicobacter pylori is more reliable than previously thought.

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References

  1. Akamatu T, Ota H, Shimizu T, Matsuzawa K, Fujimori Y, Nakamura N, Kiyosama K, Katuyama T: Histochemical study ofHelicobacter pylori and surface mucous gel layer in various gastric lesions. Acta Histochemica et Cytochemica 1995, 28: 181–185.

    Google Scholar 

  2. Shimizu T, Akamatsu T, Ota H, Katsuyama T: Immunohistochemical detection ofHelicobacter pylori in the surface mucous gel layer and its clinicopathological significance. Helicobacter 1996, 4: 197–206.

    Google Scholar 

  3. Ota H, Katsuyama T, Ishii K, Nakayama J, Shiozawa T, Tsukahara Y: A dual staining method for identifying mucins of different gastric epithelial mucous cells. Histochemical Journal 1991, 23: 22–28.

    Google Scholar 

  4. Ota, H, Katsuyama T: Alternating laminated array of two types of mucin in the human gastric surface mucous layer. Histochemical Journal 1992, 24: 86–92.

    Google Scholar 

  5. Misawa K, Kumagai T, Hosogaya S, Yamada K, Okimura S, Shimizu T, Akamatsu T, Tanaka E, Furihata K: Clinical and etiological studies of IgG antibodies toHelicobacter pylori detected by enzyme-linked immunosorbent assay. Japanese Journal of Clinical Pathology 1995, 43: 375–380.

    Google Scholar 

  6. Crabtree JE, Shallcross TM, Heatly RV, Wyatt JI: Evaluation of commercial ELISA for serodiagnosis ofHelicobacter pylori infection. Journal of Clinical Pathology 1991, 44: 326–328.

    Google Scholar 

  7. Hoek FJ, Noach LA, Rauws EAJ, Tytgat GNJ: Evaluation of the performance of commercial test kits for detection ofHelicobacter pylori antibodies in serum. Journal of Clinical Miccrbiology 1992, 30: 1525–1528.

    Google Scholar 

  8. Lelwala-Guruge, J, Nilsson I, Ljungh A, Wadstrom T: Cell surface proteins ofHelicobacter pylori as antigens in an ELISA and a comparison with three commercial ELISAs. Scandinavian Journal of Infectious Diseases 1992, 24: 457–465.

    Google Scholar 

  9. Schembri MA, Lin SK, Lambert JR: Comparison of commercial diagnostic tests forHelicobacter pylori antibodies. Journal of Clinical Microbiology 1993, 31: 2621–2624.

    Google Scholar 

  10. van den Over HLA, Loffeld RJLF, Stobberingh EE: Usefulness of a new serological test (Bio-Rad) to diagnoseHelicobacter pylori-associated gastritis. Journal of Clinical Microbiology 1991, 29: 283–286.

    Google Scholar 

  11. Aguirre PM, Pascual CY, Merino FJ, Velaso AC: Evaluation of two commercial enzyme immunoassays for the diagnosis ofHelicobacter pylori infection. European Journal of Clinical Microbiology & Infectious Diseases 1992, 11: 634–639.

    Google Scholar 

  12. Trautmann M, Moldrzky M, Vogt K, Korber J, Held T, Marre R: Use of a receiver operating characteristic in the evaluation of two commercial enzyme immunoassays for detection ofHelicobacter pylori infection. European Journal of Clinical Microbiology & Infectious Diseases 1994, 13: 812–819.

    Google Scholar 

  13. Swets JA: Measuring the accuracy of diagnostic systems. Science 1988, 240: 1285–1293.

    Google Scholar 

  14. Talley NJ, Newell DG, Ormand JE, Carpenter HA, Wilson WR, Zinsmeister AR, Perez-Perez GI, Blaster MJ: Serodiagnosis ofHelicobacter pylori: Comparison of enzymelinked immunosorbent assays. Journal of Clinical Microbiology 1991, 29: 1635–1639.

    Google Scholar 

  15. Cutler AF, Havstad S, Ma CK, Blasser MJ, Perez-Perez GI, Schubert TT: Accuracy of invasive and noninvasive tests to diagnoseHelicobacter pylori infection. Gastroenterology 1995, 109: 136–141.

    Google Scholar 

  16. Dill S, Payne-James JJ, Misiewicz JJ, Grimble GK, McSwiggan D, Pathak K, Wood AJ, Scrimgeour CM, Rennie MJ: Evaluation of13C-urea breath test in the detection ofHelicobacter pylori and in monitoring the effect of tripotassium dicitratobismuthate in non-ulcer dyspepsia. Gut 1990, 31: 1237–1241.

    Google Scholar 

  17. Graham DY, Klein PD, Evans DJ Jr, Evans DG, Alpert LC, Opekun AR, Boutton TW:Campylobacter pylori detected noninvasively by the13C-urea breath test. Lancet 1987, i: 1174–1177.

    Google Scholar 

  18. Feldman RA, Deeks JJ, Evans SJ, and theHelicobacter pylori Serology Study Group: Multi-laboratory comparison of eight commercially availablehelicobacter pylori serology kits. European Journal of Clinical Microbiology & Infectious Diseases 1995, 14: 428–433.

    Google Scholar 

  19. Cognein P, Costa A, Giacosa A: Serodiagnosis ofHelicobacter pylori: evaluation of a rapid, miniaturized immunochromatographie test. European Journal of Cancer Prevention 1994, 3: 457–463.

    Google Scholar 

  20. Jensen AKV, Andersen LP, Wachmann CH: Evaluation of eight commercial kits forHelicobacter pylori IgG antibody detection. APMIS 1994, 101: 795–801.

    Google Scholar 

  21. Gosciniak G, Matysiak-Budnik T, Przondo-Mordarska A: Evaluation of three serological tests for the detection ofHelicobacter pylori associated infection. Archivum Immunologiae et Therapiae Experimentalis 1993, 41: 315–319.

    Google Scholar 

  22. Cutler A, Schubert A, Schubert T: Role ofHelicobacter pylori serology in evaluating treatment success. Digestive Diseases and Sciences 1993, 38: 2262–2266.

    Google Scholar 

  23. Goossens H, Glupczynski Y, Burette A, van den Borre C, DePrez C, Bodenmann J, Keller A, Butzler JP: Evaluation of commercially available complement fixation test for diagnosis ofHelicobacter pylori infection and for follow-up after antimicrobial therapy. Journal of Clinical Microbiology 1992, 30: 3230–3233.

    Google Scholar 

  24. Kosunen TU, Seppala K, Sarna S, Sipponen P: Diagnostic value of decreasing IgG, IgA and IgM antibody titers after eradication ofHelicobacter pylori. Lancet 1992, 339: 893–895.

    Google Scholar 

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Authors and Affiliations

  1. Department of Laboratory Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan

    K. Misawa, T. Kumagai, K. Furihata, H. Ota & T. Katsuyama

  2. Department of Internal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan

    T. Shimizu & T. Akamatsu

Authors
  1. K. Misawa
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  2. T. Kumagai
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  3. T. Shimizu
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  4. K. Furihata
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  5. H. Ota
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  6. T. Akamatsu
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  7. T. Katsuyama
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Misawa, K., Kumagai, T., Shimizu, T. et al. A new histological procedure for re-evaluation of the serological test forHelicobacter pylori . Eur. J. Clin. Microbiol. Infect. Dis. 17, 14–19 (1998). https://doi.org/10.1007/BF01584357

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