Abstract
AKlebsiella pneumoniae strain having mobilization “helper” potential has been isolated from the river Rhine. Analysis of the transconjugants resulting from the mobilization of nonconjugative pBR-type plasmids and RSF1010 derivatives showed that the transfer-helper capacity of theK. pneumoniae strain is related to the presence of a Tn3-like transposable element, Tn5403. This element has been identified and localized in a plasmid.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature Cited
Bagdasarian M, Lurz R, Ruckert B, Franklin FCH, Bagdasarian MM, Frey J, Timmis KN (1981) Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning inPseudomonas. Gene 16:237–247
Bennett PM, Richmond MH (1976) Translocation of a discrete piece of desoxyribonucleic acid carrying anamp gene between replicons inE. coli. J Bacteriol 126:1–6
Bennett PM, Grinsted J, Choi CL, Richmond MH (1978) Characterization of Tn501 a transposon determining resistance to mercuric ions. Mol Gen Genet 159:101–106
Clark AJ, Warren GJ (1979) Conjugal transmission of plasmids. Annu Rev Genet 13:99–125
Clewell DB, Helinski DR (1969) Supercoiled circular DNA-protein complex inEscherichia coli: purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci USA 62:1159–1166
Cohen SN, Chang ACY, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: genetic transformation ofEscherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114
Crisona NJ, Novak JA, Nagaishi H, Clark AJ (1980) Transposon-mediated conjugational transmission of non-conjugative plasmids. J Bacteriol 142:701–713
Dower WJ, Miller JF, Ragsdale CW (1988) High efficiency transformation ofE. coli by high voltage electroporation. Nucleic Acids Res 16:6127–6145
Grunstein M, Hogness D (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965
Guerry P, van Embden J, Falkow S (1974) Molecular nature of two nonconjugative plasmids carrying drug resistance genes. J Bacteriol 117:619–630
Guyer MS (1978) The γδ sequence of F is an insertion sequence. J Mol Biol 126:347–365
Hallé F, Meyer J-M (1989) Ferripyoverdine-reductase activity inPseudomonas fluorescens. Biol Metals 2:18–24
Henschke RB, Schmidt FRJ (1990) Plasmid mobilization from genetically engineered bacteria to members of the indigenous soil microflora in situ. Curr Microbiol 20:105–110
Hill KE, Weightman AJ, Fry JC (1992) Isolation and screening of plasmids from the epilithon which mobilize recombinant plasmid pD10. Appl Environ Microbiol 58:1292–1300
Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197
McPherson P, Gealt MA (1986) Isolation of indigenous waste-water bacterial strains capable of mobilizing plasmid pBR325. Appl Environ Microbiol 51:904–909
Mirgain I, Werneburg B, Harf C, Monteil H (1989) Phenyl mercuric acetate biodegradation by environmental strains of Pseudomonas species. Res Microbiol 140:695–707
Mirgain I, Hagnere C, Green GA, Harf C, Monteil H (1992) Synthetic oligonucleotide probes for detection of mercuryresistance genes in environmental freshwater microbial communities in response to pollutants. World J Microbiol Biotech 8:30–38
Rosenberg C, Casse-Delbart F, Dusha I, David M, Boucher C (1982) Megaplasmids in the plant-associated bacteriaRhizobium meliloti andPseudomonas solanacearum. J Bacteriol 150:402–406.
Roux B, Lebaron P, Huberg J-C, Lett M-C (1993) Occurrence of transposable elements in aquatic bacteria strains: involvement in the mobilization of pBR-type plasmids. Microb Releases 1:223–228
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory Press
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci. USA 74:5463–5467
Scholz P, Haring V, Wittmann-Liebold B, Ashman K, Bagdasarian M, Scherzinger E (1989) Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene 75:271–288
Serghini A, Ritzenthaler C, Pinck L (1989) A rapid and efficient ‘miniprep’ for isolation of plasmid DNA. Nucleic Acids Res 17:3604
Sherratt D (1989) Chapter 5. Tn3 and related transposable elements: site-specific recombination and transposition. In: Berg DE, Howe MM, eds. Mobile DNA. Washington, D.C.: American Society for Microbiology, pp 163–184
Sorensen SJ (1992) Mobilization of non-conjugative pBR322-derivative plasmids from laboratory strains ofEscherichia coli to bacteria isolated from seawater. Microb Releases 1:11–16
Watanabe T, Furuse C, Sakaizumi S (1968) Transduction of various R factors by phage P1 inEscherichia coli and by phage P22 inSalmonella typhimurium. J Bacteriol 96:1791–1795
Weir JP, Moss B (1983) Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations. J Virol 46:530–537
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rinkel, M., Hubert, JC., Roux, B. et al. Identification of a new transposon Tn5403 in aKlebsiella pneumoniae strain isolated from a polluted aquatic environment. Current Microbiology 29, 249–254 (1994). https://doi.org/10.1007/BF01577436
Issue Date:
DOI: https://doi.org/10.1007/BF01577436