Skip to main content
Log in

Detection of a permeability factor produced byHaemophilus pleuropneumoniae

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Toxigenic profiles ofHaemophilus pleuropneumoniae were determined for isolates rom serotypes 1 and 5. None of the 18 strains investigated produced hemolytic or proteolytic activity. Intradermal injection of rabbits with culture supernatants induced an edematous zone (permeability factor, PF) for strains of either serotypes. The presence of this PF seemed to depend on the component freshness of the culture media, especially for isolates of serotype 5. PF activity appeared at two different time intervals: at 2–3 h of culture and at 12 h of culture. Isolates ofH. pleuropneumoniae from serotypes 1 and 5 can produce a PF that is not related to hemolytic no proteolytic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur Cited

  1. Bendixen PH, Shewen PE, Rosendal S, Wilkie BN (1981) Toxicity ofHaemophilus pleuropneumoniae for porcine lung macrophages, peripheral blood monocytes and testicular cells. Infect Immun 33:673–676

    Google Scholar 

  2. Higgins R, Lariviere S, Mittal KR, Desrosiers R (1982) La recherche sur la pleuropneumonie porcine à la Faculté de Médecine vétérinaire. Med Vet Quebec 12:40–42

    Google Scholar 

  3. Higgins R, Lariviere S, Mittal KR, Martineau GP (1983) Control measures for porcine pleuropneumonia in Quebec. In: Proceedings of the World Association of Veterinary Laboratory Diagnosticians. Ames IA, pp 421–427

  4. Martin PG, Lachance P, Niven DF (1985) Production of RNA-dependent hemolysin byHaemophilus pleuropneumoniae. Can J Microbiol 31:456–462

    Google Scholar 

  5. Maudsley JR, Kadis S, Mayberry WR (1986) Isolation, purification, and partial characterization of a lipopolysaccharide fromHaemophilus pleuropneumoniae. Infect Immun 51:501–506

    Google Scholar 

  6. Mittal KR, Higgins R, Lariviere S (1983) Identification and serotyping ofHaemophilus pleuropneumoniae by coaggulation test. J Clin Microbiol 18:1351–1354

    Google Scholar 

  7. Mittal KR, Higgins R, Lariviere S, Martineau GP (1985) Use of co-agglutination for direct detection and serotyping ofHaemophilus pleuropneumoniae. In: Schultz RA (ed) Compendium of the annual meeting of the American Association of Swine Practitioners. Des Moines IA, pp 28–33

  8. Nakai T, Sawata A, Kume K (1983) Characterization of the hemolysin produced byHaemophilus pleuropneumoniae. Am J Vet Res 44:344–347

    Google Scholar 

  9. Nakai T, Sawata A, Kume K (1984) Pathogenicity ofHaemophilus pleuropneumoniae for laboratory animals and possible role of its hemolysin for production of pleuropneumonia. Jpn J Vet Sci 46:851–858

    Google Scholar 

  10. Nielsen R (1976) Pleuropneumonia of swine caused byHaemophilus parahaemolyticus. N Vet Med 28:337–348

    Google Scholar 

  11. Nielsen R, O'Connor PJ (1984) Serological characterization of eightHaemophilus pleuropneumoniae strains and proposal of a new serotype 8. Acta Vet Scand 25:96–106

    Google Scholar 

  12. Rosendal S, Boyd DA, Gilbride KA (1985) Comparative virulence of porcineHaemophilus bacteria. Can J Comp Med 49:68–74

    Google Scholar 

  13. Rosendal S, Mitchell WR, Weber M, Wilson MR, Zaman MR (1980)Haemophilus pleuropneumoniae: lung lesions induced by sonicated bacteria and sterile culture supernatant. Proc Int Pig Vet Soc Congress 5:221

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lallier, R., Le Blanc, L., Morrissette, F. et al. Detection of a permeability factor produced byHaemophilus pleuropneumoniae . Current Microbiology 15, 141–144 (1987). https://doi.org/10.1007/BF01577262

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01577262

Keywords

Navigation