Abstract
An M-like protein fromStreptococcus pyogenes type M15 strain EF1949 (EMML15) was cloned inEscherichia coli and sequenced. Recombinant EMML15 protein revealed a unique binding pattern for human IgG subclasses not described previously. Comparative analysis of the EMML15 amino acid sequence with those of other M-like proteins of opacity factor positive (OF+) serotypes and protein H, an IgG receptor from OF− serotype M1, showed that IgG-binding proteins with common binding of IgG3 were closely related and distinct from streptococcal IgG receptors not binding IgG3. Thus, the Ig-binding proteins fromS. pyogenes were subdivided into two main categories according to binding pattern, protein structure, and gene location.
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Literature Cited
Andersen JK (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Method 10:203–209
Bessen D, Fischetti VA (1990) A human IgG receptor of group A streptococci is associated with tissue site of infection and streptococcal class. J Infect Dis 161:747–754
Bessen D, Fischetti VA (1992) Nucleotide sequences of two adjacent M or M-like protein genes of group A streptococci: different RNA transcript levels and identification of a unique immunoglobulin A-binding protein. Infect Immun 60:124–135
Boyle MDP, Reis KJ (1987) Bacterial Fc receptors. Biotechnology 5:697–703
Burova LA, Schalén C, Koroleva IV, Svensson M-L (1989) Role of group A streptococcal IgG Fc-receptor in induction of anti-IgG by immunization in the rabbit. FEMS Microbiol Immunol 47:443–448
Christensen P, Schalén C (1990a) Group A streptococcal immunoglobulin A Fc receptor. In: Boyle MDP (ed) Bacterial immunoglobulin binding proteins. Orlando: Acad Press Inc., pp. 187–192
Fischetti VA (1989) Streptococcal M protein: molecular design and biological behaviour. Clin Microbiol Rev 2:285–314
Frithz E, Hedén L-O, Lindahl G (1989) Extensive sequence homology between IgA receptor and M proteins inStreptococcus pyogenes. Mol Microbiol 3:1111–1119
Gomi H, Hozumi T, Hattori S, Tagawa C, Kishimoto F, Björck L (1990) The gene sequence and some properties of protein H, a novel IgG-binding protein. J Immunol 144:4046–4052
Grubb A, Grubb R, Christensen P, Schalén C (1982) Isolation and some properties of an IgG binding protein from group A streptococci type 15. Int Arch Allergy Appl Immunol 67:369–376
Haanes EJ, Cleary PP (1989) Identification of a divergent M protein gene and an M protein-related gene family inStreptococcus pyogenes serotype 49. J Bacteriol 171:6397–6408
Heath DG, Cleary PP (1989) Fc-receptor and M protein genes of group A streptococci are products of gene duplication. Proc Natl Acad Sci USA 86:4741–4745
Hollingshead SK, Readdy TL, Yung DL, Bessen DE (1993) Structural heterogeneity of theemm gene cluster in group A streptococci. Mol Microbiol 8:707–717
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lancefield RC (1962) Current knowledge of type-specific antigens of group A streptococci. J Immunol 89:307–313
Lindahl G, Stenberg L (1990) Binding of IgA and/or IgG is a common property of clinical isolates of group A streptococci. Epidem Inf 105:87–93
Maniatis T, Frisch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press
Messing J (1983) New vectors for cloning. Methods Enzymol 101:20–78
O'Toole P, Stenberg L, Rissler M, Lindahl G (1992) Two major classes in the M protein family in group A streptococci. Proc Natl Acad Sci USA 89:1–5
Reader R, Otten RA, Chamberlin L, Boyle MDP (1992) Functional and serological analysis of type II immunoglobulin G-binding proteins expressed by pathogenic group A streptococci. J Clin Microbiol 30:3074–3081
Retnoningrum DS, Podbielski A, Cleary PP (1993) Type M12 protein fromStreptococcus pyogenes is a receptor for IgG3. J Immunol 150:2332–2340
Sanger F, Nicklen SN, Cadson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schalén C, Truedsson L, Christensen P, Christensen KK (1985) Blocking of antibody complement-dependent effector functions by streptococcal IgG Fc-receptor and staphylococcal protein A. Acta Pathol Microbiol Immunol Scand Sect B, 93:395–400
Schmidt K-H, Åkesson P, Köhler W, Björck L (1992) Isolation fromStreptococcus pyogenes of extracellular M1 protein which binds to fibrinogen, albumin and IgG. In: Orefici G (ed.) New perspectives of streptococci and streptococcal infections. Zbl. Bakt. Suppl 22:206–207
Stenberg L, O'Toole P, Lindahl G (1992) Many group A streptococcal strains express two different immunoglobulin binding proteins, encoded by closely linked genes: characterization of the proteins expressed by four strains of different M-type. Mol Microbiol 6:1185–1194
Top FH, Wannamaker LW (1968) The serum opacity reaction ofStreptococcus pyogenes: demonstration of multiple, strain-specific lipoproteinase antigens. J Exp Med 127:1013–1034
Wessels MK, Moses AE, Goldberg JE, DiCesare TJ (1991) Hyaluronic acid capsule is a virulence factor of group A streptococci. Proc Natl Acad Sci USA 88:8317–8321
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Katerov, V., Schalén, C. & Totolian, A.A. M-like, immunoglobulin-binding protein ofStreptococcus pyogenes type M15. Current Microbiology 29, 31–36 (1994). https://doi.org/10.1007/BF01570188
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DOI: https://doi.org/10.1007/BF01570188