Abstract
This study was undertaken to delineate the role of cholecystokinin (CCK) in bombesin- and meal-stimulated pancreatic polypeptide (PP) secretion in seven conscious dogs by studying: (1) the stimulatory effect of similar plasma levels of CCK induced by a meal and infusions of bombesin and the synthetic CCK analog cerulein on plasma PP, and (2) the inhibition of PP secretion by the CCK-receptor antagonist CR-1409 during these three stimuli. The stimulation of PP secretion during bombesin (11.0±1.6 nM/hr) and after the meal (8.9±2.0 nM/hr) were significantly (P<0.05) greater than during infusion of the CCK analog cerulein (2.7±0.4 nM/hr). CR-1409 significantly inhibited the bombesin- and meal-stimulated PP secretion to 2.0±0.4 nM/hr (81%; P<0.05) and 3.1±1.2 nM/hr (47%; P<0.05), respectively, while the cerulein-stimulated PP release was almost abolished to 0.2±0.1 nM/hr (93%; P<0.05) by the drug. These findings point to an important role for CCK in the regulation of bombesin- and meal-stimulated PP secretion.
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Lin TM, Evans DC, Chance RE, Spray GF: Bovine pancreatic peptide: Action on gastric and pancreatic secretion in dogs. Am J Physiol 232:E311–315, 1977
Larsson LI, Sundler F, Hakanson R: Pancreatic polypeptide: A postulated new hormone: Identification of its cellular storage site by light and electron microscopic immunocytochemistry. Diabetologia 12:211–226, 1976
Greenberg GR, McCloy RF, Adrian TE, Chadwick VS, Baron JH, Bloom SR: Inhibition of pancreas and gallbladder by pancreatic polypeptide. Lancet 2:1280–1282, 1978
De Jong AJL, Singer MV, Lamers CBHW: Effect of rabbit anti-pancreatic polypeptide serum on postprandial pancreatic exocrine secretion in dogs. Gastroenterology 90:1926–1931, 1986
Yanaihara H, Yanaihara C, Mochizuki T, Iwahara K, Fujita T, Iwanaga T: Immunoreactive GRP. Peptides 2:185–191, 1981
Floyd JC, Fajans SS, Pek S, Chance RE: A newly recognized pancreatic polypeptide; plasma levels in health and disease. Recent Prog Horm Res 33:519–570, 1977
De Jong AJL, Singer MV, Lamers CBHW: Effect of pancreatic polypeptide-antiserum on bombesin-stimulated pancreatic exocrine secretion in dogs. Peptides 8:973–976, 1987
Schwartz TW, Holst JJ, Fahrenkrug J, Lindkaer Jensen S, Nielson OV, Rehfeld JF, Stadil F: Vagal, cholinergic regulation of pancreatic polypeptide secretion. J Clin Invest 61:769–781, 1978
Adrian TE, Bloom SR, Hermanson K, Iversen J: Pancreatic polypeptide, glucagon and insulin secretion from the isolated perfused canine pancreas. Diabetologia 14:413–417, 1978
Lonovics J, Guzman S, Devitt P, Hejtmancik KE, Suddith RL, Rayford PL, Thompson JC: Release of pancreatic polypeptide in humans by infusion of cholecystokinin. Gastroenterology 79:817–822, 1980
Makovec F, Bani M, Cereda R, Chiste R, Pacini MA, Revel L, Rovati LA, Rovati LC, Setnikar I: Pharmacological properties of lorglumide as a member of a new class of cholecystokinin antagonists. Drug Res 37:1265–1268, 1987
Beglinger C, Taylor IL, Grossman MI, Solomon TE: Pancreatic polypeptide inhibits exocrine pancreatic responses to six stimulants. Am J Physiol 246:G286-G291, 1984
Solomon TE, Yamada T, Elashoff J, Wood J, Beglinger C: Bioactivity of cholecystokinin analogues: CCK-8 is not more potent than CCK-33. Am J Physiol 247:G105-G111, 1984
Hildebrand P, Beglinger C, Köhler E, Setnikar I, Gyr K: Biological effects of a proglumide derivate as cholecystokinin antagonist in conscious dogs. Regul Pept 18:213–220, 1987
Jansen JBMJ, Lamers CBHW: Radioimmunoassay of cholecystokinin in human tissue and plasma. Clin Chim Acta 131:305–316, 1983
Lamers CBHW, Diemel JM, van Leer E, van Leusen R, Peetoom J: Mechanism of elevated serum pancreatic polypeptide concentrations in chronic renal failure. J Clin Endocrinol Metab 55:922–926, 1982
Beglinger C, Fried M, Whitehouse J, Jansen JB, Lamers CB, Gyr K: Pancreatic enzyme response to a liquid meal and to hormonal stimulation; correlation with plasma secretin and cholecystokinin levels. J Clin Invest 75:1471–1476, 1985
Konturek SJ, Tasler J, Cieszkowski M, Szewczyk K, Hlady M: Effect of cholecystokinin receptor antagonist on pancreatic responses to exogenous gastrin and cholecystokinin and to meal stimuli. Gastroenterology 94:1014–1023, 1988
Hosotani R, Chowdhury P, Rayford PL: L-364,718, a new CCK antagonist, inhibits postprandial pancreatic secretion and PP release in dogs. Dig Dis Sci 34:462–467, 1989
Konturek SJ, Konturek P, Bielanski W, Szewczyk K: CCK receptors in release of pancreatic polypeptide (PP) in dogs. Dig Dis Sci 34:849–856, 1989
Konturek SJ, Tasler J, Konturek JW, Cieszkowski M, Szewczyk K, Hladij M, Anderson PS: Effect of non-peptidal CCK receptor antagonist (L-364, 718) on pancreatic responses to cholecystokinin, gastrin, bombesin, and meat feeding in dogs. Gut 30:110–117, 1989
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Supported by grant 13-37-43 from the Netherlands Foundation for Medical Research MEDIGON and grant Si 228/7-1 from the Deutsche Forschungsgemeinschaft.
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Jansen, J.B.M.J., DE Jong, A.J.L., Singer, M.V. et al. Role of cholecystokinin in bombesin- and meal-stimulated pancreatic polypeptide secretion in dogs. Digest Dis Sci 35, 1073–1077 (1990). https://doi.org/10.1007/BF01537577
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DOI: https://doi.org/10.1007/BF01537577