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The coding sequence for the human 18,000-dalton hydrophobie pulmonary surfactant protein is located on chromosome 2 and identifies a restriction fragment length polymorphism

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Somatic Cell and Molecular Genetics

Abstract

The 18-kd Hydrophobic pulmonary surfactant protein (PSP-B) is a developmentally regulated protein which is important for normal lung function. A complementary DNA probe for 221 NH2 terminal amino acids of PSP-B was used to determine the chromosomal location of this gene and identify a restriction fragment length polymorphism (RFLP). Southern blot hybridization to genomic DNA isolated from a panel of human-CHO somatic cell hybrids unambiguously maps this gene to chromosome 2. Human DNA cut with BamHI yields a RFLP with variable bands at 2.8 and 2.6 kb. Since there is a relative lack of polymorphic markers for chromosome 2, this sequence may be useful in linkage analysis.

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Authors and Affiliations

  1. Department of Medicine, Division of Pulmonary Sciences, University of Colorado Health Sciences Center, USA

    Philip A. Emrie, Talia Hofmann & James H. Fisher

  2. Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord, 80206, Denver, Colorado

    Carol Jones

  3. Webb-Waring Lung Institute, 4200 East Ninth Avenue, 80262, Denver, Colorado

    Philip A. Emrie & James H. Fisher

  4. Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, 4200 E. Ninth Avenue, 80262, Denver, Colorado

    Carol Jones

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  1. Philip A. Emrie
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  2. Carol Jones
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  3. Talia Hofmann
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  4. James H. Fisher
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Emrie, P.A., Jones, C., Hofmann, T. et al. The coding sequence for the human 18,000-dalton hydrophobie pulmonary surfactant protein is located on chromosome 2 and identifies a restriction fragment length polymorphism. Somat Cell Mol Genet 14, 105–110 (1988). https://doi.org/10.1007/BF01535054

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  • DOI: https://doi.org/10.1007/BF01535054

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