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Assignment of human dihydrofolate reductase gene to band q23 of chromosome 5 and of related pseudogene ΨHD1 to chromosome 3

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Somatic Cell and Molecular Genetics

Abstract

The chromosomal location of the human dihydrofolate reductase (DHFR; EC 1.5.1.3) gene that is amplified in a methotrexate-resistant human cell line has been investigated by screening a number of human-Chinese hamster ovary cell hybrids containing terminal and interstitial deletions in human chromosome 5. A correlation of genomic blotting data with the chromosome 5 constitution of the individual hybrids has allowed the assignment of the human DHFRgene to 5q23. The present work also establishes the location of the related intronless pseudogene ΨHD1 in chromosome 3.

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Authors and Affiliations

  1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 91125, Pasadena, California

    Barry J. Maurer

  2. Department of Biological Chemistry, California College of Medicine, University of California, Irvine, 92717, Irvine, California

    Leon Carlock & John Wasmuth

  3. Division of Biology, California Institute of Technology, 91125, Pasadena, California

    Giuseppe Attardi

Authors
  1. Barry J. Maurer
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  2. Leon Carlock
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  3. John Wasmuth
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  4. Giuseppe Attardi
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Maurer, B.J., Carlock, L., Wasmuth, J. et al. Assignment of human dihydrofolate reductase gene to band q23 of chromosome 5 and of related pseudogene ΨHD1 to chromosome 3. Somat Cell Mol Genet 11, 79–85 (1985). https://doi.org/10.1007/BF01534737

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  • DOI: https://doi.org/10.1007/BF01534737

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